Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Reflections on emerging therapies for inherited retinal diseases following a community education event: A qualitative exploration.

Optometry and vision science : official publication of the American Academy of Optometry·2026

Same author

Retinoic acid drives cell fate specification, maturation and retinal regionality in human retinal organoids.

Nature communications·2026

Same author

Selecting Novel Retino-tropic AAV Capsids by Directed Evolution.

Methods in molecular biology (Clifton, N.J.)·2026

Same author

Transduction of AAV Vectors in iPSC-Derived Retinal Organoids.

Methods in molecular biology (Clifton, N.J.)·2026

Same author

Building Australia's non-animal technologies ecosystem: a national cross-sector mapping and strategic insights from the non-animal technologies network (NAT-Net).

Frontiers in toxicology·2026

Same author

A sorghum pangenome reference improves global crop trait discovery.

Nature·2026

Same journal

Genome Editing for Familial Hemophagocytic Lymphohistiocytosis: Design Principles, Challenges, and Translational Perspectives.

Human gene therapy·2026

Same journal

Lilly, Ascidian Launch Up-to-$1.9B RNA Exon Editor Collaboration.

Human gene therapy·2026

Same journal

Hyperthermia-Enhanced Adeno-Associated Virus Gene Therapy: Mechanisms, Delivery Technologies, and Translational Potential.

Human gene therapy·2026

Same journal

Adeno-Associated Virus Gene Therapy Translation: Lessons from Early Regulatory Meetings.

Human gene therapy·2026

Same journal

In Remembrance of Professor Zelig Eshhar: <i>A Life Committed to CAR-T</i>.

Human gene therapy·2026

Same journal

<i>In Vivo</i> Genome Editing Approach to Disrupt Hydroxyacid Oxidase 1 for the Treatment of Primary Hyperoxaluria Type 1.

Human gene therapy·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Sep 30, 2025

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus AAV Capsid Variants

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus AAV Capsid Variants

Published on: October 18, 2022

AAV-p40 Bioengineering Platform for Variant Selection Based on Transgene Expression.

Adrian Westhaus^1,2, Marti Cabanes-Creus¹, Timo Jonker¹

¹Translational Vectorology Unit, Children's Medical Research Institute, Faculty of Medicine and Health, The University of Sydney, Westmead, New South Wales, Australia.

Human Gene Therapy

|March 17, 2022

Summary

This summary is machine-generated.

This study validates a functional transduction (FT) method for discovering novel adeno-associated virus (AAV) variants. The mRNA-based FT selection is optimal for identifying AAV capsid variants with improved properties.

Keywords:

AAV adeno-associated viral vectors capsid bioengineering directed evolution novel vectors vector development

More Related Videos

Transfection, Selection, and Colony-picking of Human Induced Pluripotent Stem Cells TALEN-targeted with a GFP Gene into the AAVS1 Safe Harbor

Transfection, Selection, and Colony-picking of Human Induced Pluripotent Stem Cells TALEN-targeted with a GFP Gene into the AAVS1 Safe Harbor

Published on: February 1, 2015

Engineering and Evolution of Synthetic Adeno-Associated Virus AAV Gene Therapy Vectors via DNA Family Shuffling

Engineering and Evolution of Synthetic Adeno-Associated Virus AAV Gene Therapy Vectors via DNA Family Shuffling

Published on: April 2, 2012

Related Experiment Videos

Last Updated: Sep 30, 2025

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus AAV Capsid Variants

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus AAV Capsid Variants

Published on: October 18, 2022

Transfection, Selection, and Colony-picking of Human Induced Pluripotent Stem Cells TALEN-targeted with a GFP Gene into the AAVS1 Safe Harbor

Transfection, Selection, and Colony-picking of Human Induced Pluripotent Stem Cells TALEN-targeted with a GFP Gene into the AAVS1 Safe Harbor

Published on: February 1, 2015

Engineering and Evolution of Synthetic Adeno-Associated Virus AAV Gene Therapy Vectors via DNA Family Shuffling

Engineering and Evolution of Synthetic Adeno-Associated Virus AAV Gene Therapy Vectors via DNA Family Shuffling

Published on: April 2, 2012

Area of Science:

Molecular Biology
Virology
Gene Therapy

Background:

Adeno-associated virus (AAV) directed evolution is powerful for identifying novel vector variants.
Existing selection methods (replication-competent and PCR-based) can introduce biases in AAV variant selection.

Purpose of the Study:

To validate a functional transduction (FT)-based method for rapid identification of novel AAV variants.
To compare the FT platform with existing replication-competent (RC) selection strategies.
To investigate the AAV-p40 promoter for cell-type-specific AAV library selection.

Main Methods:

Comparison of FT platform with existing RC strategies.
In vivo screening in a xenograft model of human hepatocytes.
Analysis of AAV-p40 promoter activity in vitro and in vivo.

Main Results:

The mRNA-based FT selection was identified as the most optimal AAV selection method based on selection kinetics and novel capsid function.
AAV-p40 promoter exhibits ubiquitous activity.
AAV-p40 promoter can be modified for cell-type-specific expression by incorporating binding sites for silencing transcription factors.

Conclusions:

The FT method offers a rapid and optimal approach for identifying novel AAV variants.
The AAV-p40 promoter is a versatile tool for engineering cell-type-specific AAV libraries, enhancing targeted gene delivery.
This work advances AAV vector development for gene therapy applications.