Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Energy transfer leaves fingerprints in cyanine photoswitching behavior.

PLoS computational biology·2026
Same author

Single-molecule localization microscopy reveals the molecular organization of endogenous membrane receptors.

Science advances·2026
Same author

Decoding the molecular interplay of CD20 and therapeutic antibodies with fast volumetric nanoscopy.

Science (New York, N.Y.)·2025
Same author

MYC multimers shield stalled replication forks from RNA polymerase.

Nature·2022
Same author

Convex hull as diagnostic tool in single-molecule localization microscopy.

Bioinformatics (Oxford, England)·2022
Same author

Photoswitching fingerprint analysis bypasses the 10-nm resolution barrier.

Nature methods·2022
Same journal

3DICE: Interpretable 3D Cross-Modal Learning for Drug-Target Interaction Prediction and Large-Scale Drug Discovery.

Bioinformatics (Oxford, England)·2026
Same journal

KASSPer: Kinase Active Site Structure Prediction using Protein and Ligand Language Models and Its Application to Virtual Screening.

Bioinformatics (Oxford, England)·2026
Same journal

IDR searcher: a search engine solution for public image resources.

Bioinformatics (Oxford, England)·2026
Same journal

KCFtools: Rapid alignment-free method for introgression screening and GWAS using k-mer profiles.

Bioinformatics (Oxford, England)·2026
Same journal

Meta2DB: Curated shotgun metagenomic feature sets and metadata for health state prediction.

Bioinformatics (Oxford, England)·2026
Same journal

conMItion: an R package adjusting confounding factors for associations in multi-omics.

Bioinformatics (Oxford, England)·2026
See all related articles

Related Experiment Video

Updated: Sep 30, 2025

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

8.4K

LOCAN: a python library for analyzing single-molecule localization microscopy data.

Sören Doose1

  • 1Department of Biotechnology and Biophysics, Julius-Maximilians-University, Am Hubland/Biocentre, 97074 Würzburg, Germany.

Bioinformatics (Oxford, England)
|March 17, 2022
PubMed
Summary
This summary is machine-generated.

A new Python library, LOCAN, offers flexible analysis for single-molecule localization microscopy data. This open-source tool provides essential data structures and methods for routine and customized super-resolution microscopy analysis.

More Related Videos

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
08:26

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

2.7K
Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

8.6K

Related Experiment Videos

Last Updated: Sep 30, 2025

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

8.4K
Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
08:26

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

2.7K
Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

8.6K

Area of Science:

  • Biomedical research
  • Super-resolution microscopy
  • Biophysics

Background:

  • Single-molecule localization microscopy (SMLM) is a key super-resolution technique.
  • Analyzing SMLM data requires specialized software.
  • Existing platforms may lack flexibility for diverse research needs.

Purpose of the Study:

  • To introduce LOCAN, a Python library for SMLM data analysis.
  • To provide a flexible and customizable platform for localization data.
  • To facilitate routine and advanced analysis of SMLM datasets.

Main Methods:

  • Development of a Python library named LOCAN.
  • Implementation of well-defined data structures for localization data.
  • Inclusion of versatile analysis methods for SMLM data.

Main Results:

  • LOCAN offers a scriptable and notebook-compatible analysis framework.
  • The library supports both routine and customized analysis workflows.
  • Provides a robust solution for processing point-based localization data.

Conclusions:

  • LOCAN enhances the utility of super-resolution microscopy in biomedical research.
  • The open-source library promotes accessibility and customization in SMLM data analysis.
  • Facilitates efficient and flexible analysis of single-molecule localization data.