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Epha1 is a cell-surface marker for the neuromesodermal competent population.

Luisa de Lemos¹, André Dias¹, Ana Nóvoa¹

¹Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6, 2780-156 Oeiras, Portugal.

Development (Cambridge, England)

|March 17, 2022

Summary

Researchers identified Epha1 as a cell-surface marker for neuromesodermal competent (NMC) cells in mouse embryos. This discovery allows for the isolation of axial progenitor cells for studying vertebrate development.

Keywords:

Axial progenitors EMT Epha1 NM research NMC population

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Area of Science:

Developmental Biology
Genetics
Cell Biology

Background:

Vertebrate embryonic development involves sequential tissue addition from progenitor cells.
The neuromesodermal competent (NMC) region is crucial for generating neural tube and paraxial mesoderm.
Identifying specific markers for NMC cells is essential for studying axial progenitor populations.

Purpose of the Study:

To identify cell-surface markers for isolating neuromesodermal competent (NMC) cells from mouse embryonic tissues.
To investigate the role of Epha1 as a potential marker for axial progenitor cells.
To enable the recovery of physiologically active progenitor cells without genetic modification.

Main Methods:

Genetic strategy to recover NMC cell populations from mouse embryos.
Transcriptome analysis to identify cell-surface markers.
Isolation and in vitro culture of Epha1-positive cells from mouse tailbuds.
Analysis of molecular phenotypes (Sox2, Tbxt) and Epha1 expression levels.

Main Results:

Epha1 expression is localized to axial progenitor-containing regions in mouse embryos.
Epha1-positive cells isolated from the tailbud can differentiate into neural and mesodermal lineages in vitro.
Epha1 expression correlates with specific progenitor populations: low levels with notochord progenitors, high levels with paraxial mesoderm progenitors.
Epha1-positive cells exhibit a Sox2+/Tbxt+ molecular phenotype, characteristic of NMC cells.

Conclusions:

Epha1 serves as a reliable cell-surface marker for identifying and isolating neuromesodermal competent (NMC) cells.
Epha1 expression levels can distinguish between different axial progenitor populations.
This finding provides a valuable tool for studying vertebrate axial development and progenitor cell biology.