Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Lipid Metabolism01:24

Overview of Lipid Metabolism

2.8K
Lipid metabolism is a crucial process in the human body that involves the synthesis and degradation of lipids. This process is essential for energy production, cell membrane formation, and hormone production, among other functions.
Lipolysis: The Breakdown of Lipids:
Lipolysis is the process of breaking down lipids, particularly triglycerides, into glycerol and fatty acids. This process typically occurs in the adipose tissue and is triggered by various hormones, including glucagon and...
2.8K
Lipid-derived Compounds in the Human Body01:31

Lipid-derived Compounds in the Human Body

5.4K
Fats and lipids are crucial components in the human body. Some lipid-derived compounds, such as fat-soluble vitamins, eicosanoids, lipoproteins, and glycolipids, also play unique roles to support various  biological processes .
Fat-soluble Vitamins
Fat-soluble vitamins, including vitamins A, D, E, and K, are required in minimal quantities, but their deficiencies can lead to severely abnormal physiological conditions. For example, vitamin A deficiency can cause night blindness, dry skin,...
5.4K
What are Lipids?01:38

What are Lipids?

209.7K
Overview
209.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preclinical Assessment and Tissue Distribution of Vodobatinib, a Third Generation BCr-Abl 1 Inhibitor.

Drug research·2026
Same author

Validation of a Dried Blood Spot Method for the Determination of a Third-Generation Bcr-Abl 1 Inhibitor, Vodobatinib, in Small Volumes of Rat Blood: Application to a Pharmacokinetic Study.

Biomedical chromatography : BMC·2025
Same author

Validated HPLC-UV Method for the Quantification of a Novel BCr-Abl 1 Inhibitor, Vodobatinib, in Rat Plasma: Application to a Pharmacokinetic Study.

Biomedical chromatography : BMC·2025
Same author

Highly Sensitive LC-MS/MS Method for Determination of Dexamethasone in Rat Plasma and Brain Tissue: An Application to Pharmacokinetic Study in Rats.

Biomedical chromatography : BMC·2024
Same author

Validated HPLC-UV method for quantification of paxalisib, a pan PI3K and mTOR inhibitor in mouse plasma: Application to a pharmacokinetic study in mice.

Biomedical chromatography : BMC·2023
Same author

Validated LC-MS/MS method for the determination of copanlisib in mouse dried blood spots.

Biomedical chromatography : BMC·2022

Related Experiment Video

Updated: Sep 30, 2025

Shotgun Lipidomics of Rodent Tissues
11:46

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

2.2K

A concise review on lipidomics analysis in biological samples.

Ramani Venkata Addepalli1, Ramesh Mullangi2

  • 1Palm Meadows, Kompally, Hyderabad-500100, India.

ADMET & DMPK
|March 18, 2022
PubMed
Summary

Lipidomics analyzes complex biological samples to identify lipid species. This review covers sample preparation, mass spectrometry, and ion mobility techniques for accurate lipid analysis in health and disease.

Keywords:
LC-MS/MSLipidsbioanalysissamples processing

More Related Videos

Lipidomics and Transcriptomics in Neurological Diseases
09:58

Lipidomics and Transcriptomics in Neurological Diseases

Published on: March 18, 2022

3.6K
Sample Preparation for Rapid Lipid Analysis in Drosophila Brain Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging
09:00

Sample Preparation for Rapid Lipid Analysis in Drosophila Brain Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging

Published on: July 14, 2022

3.3K

Related Experiment Videos

Last Updated: Sep 30, 2025

Shotgun Lipidomics of Rodent Tissues
11:46

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

2.2K
Lipidomics and Transcriptomics in Neurological Diseases
09:58

Lipidomics and Transcriptomics in Neurological Diseases

Published on: March 18, 2022

3.6K
Sample Preparation for Rapid Lipid Analysis in Drosophila Brain Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging
09:00

Sample Preparation for Rapid Lipid Analysis in Drosophila Brain Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging

Published on: July 14, 2022

3.3K

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Lipids are crucial molecules involved in biological activities and disease processes.
  • Lipidomics aims to comprehensively identify and quantify lipid species within biological systems.
  • Analyzing lipids in complex biological samples like tissues and fluids presents significant challenges due to sample complexity and the presence of isomers.

Purpose of the Study:

  • To provide an overview of the lipidomics workflow.
  • To discuss various analytical techniques used in lipidomics.
  • To highlight state-of-the-art mass spectrometry and ion mobility spectroscopy for lipid analysis.

Main Methods:

  • Sample preparation techniques for lipid extraction and analysis.
  • Mass spectrometry (MS) approaches, including shotgun lipidomics and liquid chromatography-MS (LC-MS).
  • Ion mobility spectroscopy (IMS) coupled with MS for resolving isomers and isobars.
  • Advanced imaging techniques for spatial lipid analysis.

Main Results:

  • Detailed review of established and emerging lipidomics methodologies.
  • Discussion of the strengths and limitations of various analytical techniques.
  • Examples illustrating the application and power of different lipidomics approaches.
  • Strategies for overcoming challenges in lipidomic analysis, such as isomer separation.

Conclusions:

  • Accurate lipidomic analysis requires careful consideration of sample preparation and analytical techniques.
  • Mass spectrometry and ion mobility spectroscopy are powerful tools for comprehensive lipid profiling.
  • Addressing the challenges of sample complexity and isomer identification is key to advancing lipidomics research.