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On 'Tissue sulfhydryl groups' by George L.Ellman.

Holly R Ellis1

  • 1Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina University, USA.

Archives of Biochemistry and Biophysics
|March 18, 2022
PubMed
Summary

George L. Ellman

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Proteomics

Background:

  • The quantification of thiols is crucial in biological and chemical research.
  • Ellman's seminal 1959 work provided a key method for thiol analysis.

Purpose of the Study:

  • To commemorate the 1959 publication by George L. Ellman on thiol quantification.
  • To highlight the enduring impact of Ellman's Reagent (DTNB) in scientific research.

Main Methods:

  • Utilized 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) for thiol quantification.
  • Applied the method to analyze thiols in biological tissues and purified proteins.

Main Results:

  • Established a reliable spectrophotometric method for determining thiol concentrations.
  • Demonstrated the reagent's efficacy across various biological samples.

Conclusions:

  • Ellman's method remains a foundational technique in biochemistry and related fields.
  • The DTNB assay continues to be widely referenced and applied in diverse scientific disciplines.
Keywords:
DTNBProteinsThiols

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