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Updated: Sep 29, 2025

Loop-mediated Isothermal Amplification LAMP Assays for the Species-specific Detection of Eimeria that Infect Chickens
Published on: February 20, 2015
Avian leukosis virus (ALV) is highly prevalent in fancy-chicken flocks in Saxony
Markus Freick1, Ruben Schreiter2, Jim Weber3
1Faculty Agriculture/Environment/Chemistry, HTW Dresden-University of Applied Sciences, Pillnitzer Platz 2, 01326, Dresden, Germany. markus.freick@htw-dresden.de.
Abstract:
The current prevalence of avian leukosis virus (ALV) in fancy chickens in Germany is unknown. Therefore, 537 cloacal swabs from 50 purebred fancy-chicken flocks in Saxony were tested for the presence of the ALV p27 protein using a commercial antigen-capture ELISA. The detection rate was 28.7% at the individual-animal level and 56.0% at the flock level. Phylogenetic analysis of PCR products obtained from 22 different flocks revealed the highest similarity to ALV subtype K. When classifying breeds by their origin, ALV detection rates differed significantly. Evaluation of questionnaire data revealed no significant differences between ALV-positive and negative flocks regarding mortality.
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