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NF-kB pathway is involved in microscopic colitis pathogenesis

Laura Francesca Pisani1, Gianeugenio Tontini2,3, Maurizio Vecchi2,3

  • 1Gastroenterology and Endoscopy Unit, IRCCS Policlinico San Donato, San Donato Milanese, Italy.

The Journal of International Medical Research
|March 18, 2022
PubMed
Abstract

Insights

Microscopic colitis (MC) development involves impaired non-canonical nuclear transcription factor kappa B (NF-kB) inflammatory pathways. This study identified increased expression of specific NF-kB pathway genes in MC patients compared to healthy controls.

Area of Science:

  • Gastroenterology
  • Immunology
  • Molecular Biology

Background:

  • Microscopic colitis (MC) is an inflammatory bowel disease.
  • The precise inflammatory pathways driving MC pathogenesis remain incompletely understood.

Purpose of the Study:

  • To investigate the inflammatory pathways implicated in the development of microscopic colitis (MC).

Main Methods:

  • Prospective analysis of human intestinal tissues from healthy controls (HC), MC patients, and ulcerative colitis (UC) patients.
  • Utilized RT2 Profiler PCR Array to assess 84 inflammatory and autoimmunity genes.
  • Validated findings using real-time polymerase chain reaction (PCR) on independent MC samples.

Main Results:

  • Gene expression analysis revealed significantly increased levels of C-C motif chemokine ligand 19, C-C motif chemokine ligand 21, lymphotoxin beta, and complement C3 in MC patients compared to HC.
  • These upregulated genes are integral components of the non-canonical nuclear transcription factor kappa B (NF-kB) pathway.
  • Real-time PCR confirmed these elevated gene expression patterns in MC.

Conclusions:

  • The study findings indicate a potential impairment in the non-canonical NF-kB pathway contributes to the development of microscopic colitis.
  • This suggests the non-canonical NF-kB pathway as a potential therapeutic target for MC.
Keywords:
Microscopic colitiscollagenous colitisinflammationinflammatory pathwaylymphocytic colitis

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