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A simple and rapid method to assay SARS-CoV-2 RNA based on a primer exchange reaction
Dayong Li1, Chengjie Duan1, Wenting Cheng1
1State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, P. R. China. xiangy@nju.edu.cn.
Abstract:
A simple method is proposed in this work for the detection of SARS-CoV-2 RNA based on a primer exchange reaction (PER). By ingeniously integrating the PER cascade and CRISPR/cas12a system, this method can achieve convenient detection of the target RNA in 40 min and distinguish a single-base mutation from the target sequence, demonstrating its superior analytical performance.

