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Developmental modulation of neuronal cell surface determinants.
Bibliotheca Anatomica
|January 1, 1986
Summary
Cell surface receptors on developing neurons change during differentiation. These changes, including lectin and antigen expression, influence neuronal reaggregation and migration in the cerebral cortex.
Area of Science:
- Neuroscience
- Developmental Biology
- Cell Biology
Background:
- Cellular differentiation involves structural changes in cell membranes.
- Neuronal migration and differentiation are critical processes in cerebral cortex development.
Purpose of the Study:
- To analyze cell surface receptor expression during pre- and perinatal neuronal development.
- To investigate the role of these surface structures in neuronal reaggregation and migration.
Main Methods:
- Analysis of antigen and lectin receptor expression on neuronal cell surfaces.
- In vitro reaggregation assays of dissociated neurons.
- Blockade of cell surface structures using monoclonal antibodies and saccharides.
Main Results:
- Some cell surface structures (e.g., RPL, PWL, ConA receptors, MHC antigens) are consistently expressed.
- Expression of other structures (e.g., LPL receptors, Thy-1) varies with developmental stage.
- Receptor expression patterns differ between proliferating, migrating, and post-migratory neurons.
- Neuronal reaggregation is calcium-dependent and influenced by temperature and cell contact frequency.
Conclusions:
- Cell surface receptor modulation, potentially enzyme-mediated, accompanies neuronal differentiation and migration.
- Specific cell surface structures influence neuronal reaggregation and cell-cell interactions.