Related Experiment Video

Updated: Sep 29, 2025

Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80
12:28

Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80

Published on: May 3, 2015

12.3K

Degradation of NSD3: What to Myc of it all?

Adam I Green1, George M Burslem2

  • 1Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.

Cell Chemical Biology
|March 18, 2022
PubMed

Insights

Researchers developed novel proteolysis targeting chimeras to degrade NSD3, a key protein in cancer. This approach suppresses oncogene transcription, offering a new therapeutic strategy for acute myeloid leukemia.

Area of Science:

  • Oncology
  • Molecular Biology
  • Drug Discovery

Background:

  • Uncontrolled gene expression drives cancer development.
  • Targeting oncogenes like cMyc is crucial for cancer therapy.
  • Acute myeloid leukemia (AML) requires novel therapeutic strategies.

Purpose of the Study:

  • To develop novel therapeutics for cancer treatment.
  • To investigate the role of NSD3 in oncogene transcription.
  • To suppress cMyc-related oncogene transcription in AML.

Main Methods:

  • Design and synthesis of proteolysis targeting chimeras (PROTACs).
  • Induction of NSD3 protein degradation.
  • Assessment of cMyc-related oncogene transcription suppression.
  • Utilizing a model of acute myeloid leukemia.

Main Results:

  • Successfully developed PROTACs targeting NSD3.
  • PROTACs induced significant degradation of NSD3 protein.
  • Demonstrated suppression of cMyc-related oncogene transcription.
  • Therapeutic potential shown in an AML model.

Conclusions:

  • PROTACs targeting NSD3 are effective in degrading the target protein.
  • This strategy suppresses oncogene transcription in AML.
  • NSD3 degradation represents a promising therapeutic avenue for AML and potentially other cancers.

More Related Videos

Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene08:18

Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene

Published on: December 31, 2014

28.2K
Assays for the Degradation of Misfolded Proteins in Cells10:56

Assays for the Degradation of Misfolded Proteins in Cells

Published on: August 28, 2016

12.2K

Related Concept Videos

Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
10.9K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
7.9K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
5.8K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.7K