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Updated: Sep 29, 2025
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
Published on: May 25, 2018
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"X" marks the spot: Mining the gold in CasX for gene editing
1Institute of Molecular Biophysics, Florida State University, Tallahassee, FL 32306, USA.
Molecular Cell
|March 18, 2022
Abstract
None:
In this issue of Molecular Cell, Tsuchida et al. (2022) present a successful structure-guided effort in improving genome-editing efficiencies of CRISPR-CasX from Deltaproteobacteria (DpbCasX) and Planctomycetes (PlmCasX). Engineered variants that stabilize the active conformational state improved the catalytic efficiency by ∼10-20 fold in vitro and mean-editing efficiency by ∼2-3 fold in human cells.
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