Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

15.2K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
15.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Plug-in hybrid baculovirus expression vector for high-yield recombinant adeno-associated virus gene therapy vector production.

Trends in biotechnology·2026
Same author

Productivity and genetic stability of a novel baculovirus vector for multigene expression from independent transgene loci.

Molecular therapy. Advances·2026
Same author

Teixobactin: A Resistance-Evading Antibiotic for Treating Anthrax.

ACS infectious diseases·2025
Same author

Amyloid-β-Driven Synaptic Deficits Are Mediated by Synaptic Removal of GluA3-Containing AMPA Receptors.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2025
Same author

Celebrating the Birthday of AMPA Receptor Nanodomains: Illuminating the Nanoscale Organization of Excitatory Synapses with 10 Nanocandles.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2024
Same author

Axonal endoplasmic reticulum tubules control local translation via P180/RRBP1-mediated ribosome interactions.

Developmental cell·2024

Related Experiment Video

Updated: Sep 29, 2025

Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons
14:02

Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons

Published on: October 31, 2020

5.9K

A coordinate-based co-localization index to quantify and visualize spatial associations in single-molecule

Jelmer Willems1, Harold D MacGillavry2

  • 1Division of Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, 3584 CH, Utrecht, The Netherlands.

Scientific Reports
|March 19, 2022
PubMed
Summary
This summary is machine-generated.

A new algorithm quantifies protein co-localization using super-resolution microscopy data. This method accurately measures protein associations in biological samples, aiding the study of protein complexes.

More Related Videos

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis
07:48

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

Published on: July 3, 2015

8.9K
Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

10.4K

Related Experiment Videos

Last Updated: Sep 29, 2025

Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons
14:02

Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons

Published on: October 31, 2020

5.9K
Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis
07:48

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

Published on: July 3, 2015

8.9K
Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

10.4K

Area of Science:

  • Cell Biology
  • Microscopy Techniques
  • Biophysics

Background:

  • Understanding protein complex organization and interactions is crucial in cell biology.
  • Super-resolution microscopy, like single-molecule localization microscopy (SMLM), offers nanoscale resolution for protein distribution.
  • Existing quantitative methods for SMLM data are limited, necessitating new analytical approaches.

Purpose of the Study:

  • To develop and validate a novel algorithm for quantifying co-localization in dual-color SMLM datasets.
  • To provide a robust and broadly applicable method for analyzing molecular spatial associations.
  • To facilitate the study of protein interactions and complex organization using super-resolution imaging.

Main Methods:

  • A local density-based algorithm was developed to analyze dual-color SMLM data.
  • The algorithm requires only molecular coordinates and localization precision as input.
  • Method validation was performed using simulated point patterns and experimental SMLM imaging.

Main Results:

  • The algorithm robustly quantifies co-localization in SMLM datasets, independent of localization density.
  • The method demonstrates high sensitivity towards local protein enrichments.
  • Validation using microtubule networks and neuronal synapses confirmed its broad applicability.

Conclusions:

  • A simple, user-friendly algorithm for analyzing dual-color SMLM data has been presented.
  • This method enhances the quantitative analysis of molecular spatial associations from super-resolution microscopy.
  • The tool aids in understanding protein complex organization and interactions in biological systems.