Polylactic acid film surface functionalized by zwitterionic poly[2-(methacryloyloxy)ethyl choline phosphate] with improved biocompatibility

Polylactic acid (PLA) is a non-toxic, biodegradable biological material that is widely used in tissue engineering and regenerative medicine. PLA is easy to adsorb non-specific proteins and lacks cell adhesion after implantation. Choline phosphate (CP) is a novel zwitterion with a reverse structure of phosphate choline (PC) on the cell membrane that can form a specific "CP-PC" interaction to promote cell adhesion. In our previous work, modification of choline phosphate polymers (PMCP) onto the PLA film surface improved the hydrophilicity and degradation properties. In this study, we further investigated the biocompatibility of PLA-PMCP films from protein adsorption, cell adhesion and proliferation, bacterial adhesion, blood compatibility, and inflammation in vivo. The PLA-PMCP surface can resist protein adsorption and bacterial adhesion due to the anti-fouling properties of the zwitterion PMCP. Meanwhile, the PLA-PMCP surface promotes the adhesion and proliferation of BMSCs due to the specific "CP-PC" effect. In addition, the PLA-PMCP film has good blood compatibility as well as the PLA film. During in vivo experiments, biocompatibility was improved and the inflammatory response and immune rejection of PLA-PMCP films were reduced compared to those of the original PLA film. Therefore, the PMCP-modified PLA film resists protein adsorption and bacterial adhesion, promotes cell adhesion and proliferation, and has good hemocompatibility and histocompatibility. This brings a significant potential for application in the fields of tissue engineering and regenerative medicine.