Polylactic acid film surface functionalized by zwitterionic poly[2-(methacryloyloxy)ethyl choline phosphate] with...

Area of Science:

• Biomaterials Science
• Tissue Engineering
• Surface Chemistry

Background:

• Polylactic acid (PLA) is a biodegradable material used in regenerative medicine but suffers from poor cell adhesion and protein adsorption.
• Choline phosphate (CP) is a zwitterion that can interact with cell membranes to enhance cell adhesion.
• Previous work showed that modifying PLA with choline phosphate polymers (PMCP) improves hydrophilicity and degradation.

Purpose of the Study:

• To investigate the biocompatibility of PLA-PMCP films.
• To evaluate protein adsorption, cell adhesion, bacterial adhesion, blood compatibility, and in vivo inflammatory response.
• To assess the potential of PLA-PMCP for tissue engineering applications.

Main Methods:

• Modification of PLA film surface with PMCP.
• Assessment of protein adsorption and bacterial adhesion on PLA and PLA-PMCP surfaces.
• Evaluation of bone marrow stromal cell (BMSC) adhesion and proliferation.
• Testing of blood compatibility and in vivo inflammatory response in animal models.

Main Results:

• PLA-PMCP surfaces exhibited reduced protein and bacterial adhesion due to PMCP's anti-fouling properties.
• The PLA-PMCP surface promoted BMSC adhesion and proliferation via specific 'CP-PC' interactions.
• PLA-PMCP films demonstrated good blood compatibility, similar to unmodified PLA.
• In vivo studies showed improved biocompatibility, reduced inflammation, and less immune rejection for PLA-PMCP compared to PLA.

Conclusions:

• PMCP modification enhances PLA's biocompatibility by resisting fouling and promoting cell integration.
• The PLA-PMCP film shows significant potential for applications in tissue engineering and regenerative medicine.
• The specific 'CP-PC' interaction is key to improved cell adhesion and overall biocompatibility.