Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inflammatory Response II: Inflammatory Exudate and Tissue Repair01:24

Inflammatory Response II: Inflammatory Exudate and Tissue Repair

5.9K
The immune system's inflammatory response destroys the invading pathogen, permitting the tissue to heal. The changes during the cellular and vascular stages allow exudate formation at the site of inflammation. The inflammatory exudate released from the wound has high protein content and a specific gravity above 1.020.
The typical wound exudate is odorless, transparent, straw-colored, thin, and watery. Exudate, however, can differ depending on the state of wound healing. Likewise, the...
5.9K
Inflammatory Response01:28

Inflammatory Response

9.6K
An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
9.6K
Inflammatory Response I: Vascular and Cellular01:30

Inflammatory Response I: Vascular and Cellular

12.8K
The inflammatory response is the body's defense against infection, injury, or irritation from bacteria, trauma, toxins, or heat. Inflammation helps locate and destroy pathogens and remove damaged tissue elements to heal the body. During this initial phase, fluid, blood products, and nutrients migrate to the injured area, resulting in redness, heat, swelling, ache, and loss of function. Moreover, signs of systemic inflammation include fever, increased WBC count, malaise, anorexia, nausea,...
12.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

miRNA family miR-29 inhibits PINK1-PRKN signaling via ATG9A.

Molecular neurodegeneration advances·2026
Same author

Phylogenetic Analysis and Structural Evaluation of <i>Staphylococcus aureus</i> Serine-Aspartate Repeat-Containing Protein D with a Focus on Periprosthetic Joint Infection.

bioRxiv : the preprint server for biology·2026
Same author

Resistance to novel β-lactam/β-lactamase inhibitors among carbapenem-resistant <i>Pseudomonas aeruginosa</i> and clinical implications in the prospective observational <i>Pseudomonas</i> study.

Antimicrobial agents and chemotherapy·2026
Same author

Escherichia coli ST131 Drives Carbapenem Use for E. Coli Bloodstream Infections.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·2026
Same author

Caregiver Communication and Preparedness During Transitions in Care: Results from a Randomized Controlled Trial.

Journal of palliative medicine·2025
Same author

Activity of contezolid against methicillin-resistant <i>Staphylococcus aureus</i> in a rat model of foreign body osteomyelitis.

Microbiology spectrum·2025

Related Experiment Video

Updated: Sep 29, 2025

Author Spotlight: Advancing Research on Candida albicans Biofilm-Associated Prosthetic Joint Infections
04:37

Author Spotlight: Advancing Research on Candida albicans Biofilm-Associated Prosthetic Joint Infections

Published on: February 2, 2024

968

Human transcriptomic response to periprosthetic joint infection.

Thao L Masters1, Aditya V Bhagwate2, Mrunal K Dehankar2

  • 1Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, United States.

Gene
|March 20, 2022
PubMed
Summary

This study identified novel gene biomarkers for diagnosing periprosthetic joint infection (PJI) and differentiating between bacterial causes. These findings offer new diagnostic targets for PJI, improving patient outcomes.

Keywords:
BiomarkersHost responsePeriprosthetic joint infectionRNA-SeqSonicate fluid

More Related Videos

Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction
10:35

Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction

Published on: December 3, 2017

11.3K
A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials
06:18

A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials

Published on: March 3, 2023

1.5K

Related Experiment Videos

Last Updated: Sep 29, 2025

Author Spotlight: Advancing Research on Candida albicans Biofilm-Associated Prosthetic Joint Infections
04:37

Author Spotlight: Advancing Research on Candida albicans Biofilm-Associated Prosthetic Joint Infections

Published on: February 2, 2024

968
Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction
10:35

Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction

Published on: December 3, 2017

11.3K
A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials
06:18

A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials

Published on: March 3, 2023

1.5K

Area of Science:

  • Orthopedics
  • Infectious Diseases
  • Molecular Biology

Background:

  • Periprosthetic joint infection (PJI) is a severe complication of joint replacement surgery.
  • Accurate diagnosis of PJI can be challenging, distinguishing it from other causes of arthroplasty failure.
  • Understanding the molecular mechanisms of PJI is crucial for developing effective diagnostic tools.

Purpose of the Study:

  • To identify and validate novel gene expression biomarkers for the diagnosis of PJI.
  • To compare gene expression profiles in PJI versus non-infectious arthroplasty failure.
  • To investigate differences in gene expression based on causative bacterial species.

Main Methods:

  • Gene expression profiling of 93 human arthroplasty specimens.
  • Differential gene expression analysis comparing infected and non-infected samples.
  • Comparative analysis of infections caused by staphylococci versus other bacteria, and specific Staphylococcus species.

Main Results:

  • Confirmed 28 known potential PJI biomarkers and identified three novel candidates: CCL20, F7, and FCRL4.
  • Observed distinct gene expression patterns for staphylococcal infections (IL13, IL17D, MMP3) and S. aureus versus S. epidermidis infections (IL1B, IL8, PF4V1).
  • Pathway analysis indicated activation of immune response and cellular repair mechanisms.

Conclusions:

  • Novel gene biomarkers (CCL20, F7, FCRL4) show potential for PJI diagnosis.
  • Specific gene expression profiles can help differentiate PJI caused by different bacterial pathogens.
  • These findings provide new molecular targets for PJI diagnosis and pathogen identification.