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A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response

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ARR17 controls dioecy in Populus by repressing B-class MADS-box gene expression.

Ana P Leite Montalvão¹, Birgit Kersten¹, Gihwan Kim¹

¹Thünen Institute of Forest Genetics, Sieker Landstrasse 2, 22927 Grosshansdorf, Germany.

Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences

|March 21, 2022

Summary

This summary is machine-generated.

The sex determinant gene ARR17 in aspen controls flower development through a small genetic network, not cytokinin signaling. This highlights minimal genetic differences between male and female aspen, impacting floral organ development.

Keywords:

cytokinin dioecy flower development poplar single gene sex determination

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Published on: July 23, 2014

Area of Science:

Plant genetics
Molecular biology
Evolutionary biology

Background:

Genetic basis of sex determination is increasingly understood in dioecious species.
Molecular mechanisms downstream of sex determinants remain largely unknown.
Aspen (Populus tremula) is a model for studying sex determination.

Purpose of the Study:

Investigate the molecular mechanisms controlled by the sex determinant gene ARR17 in aspen.
Determine if ARR17 functions in cytokinin signaling or directly in floral development.
Identify downstream genes regulated by ARR17.

Main Methods:

RNA-sequencing of isogenic aspen lines differing in ARR17.
CRISPR-Cas9 gene editing to create arr17 knockout mutants.
Analysis of gene expression changes related to floral development.

Main Results:

ARR17 regulates a narrow genetic network controlling female and male flower development.
CRISPR-Cas9-mediated arr17 knockout affected a small number of genes, suggesting a specific role.
Key floral development genes, UNUSUAL FLORAL ORGANS (UFO) and PISTILLATA (PI), were de-repressed in arr17 mutants.
ARR17's role appears to be independent of cytokinin (CK) hormone signaling.

Conclusions:

The poplar response regulator ARR17 plays a CK-independent role in floral development.
Minimal genetic differences exist between female and male aspen individuals.
This study advances understanding of sex determination and floral organogenesis in plants.