Chemical characterization, thrombolytic and antioxidant activity of Hibiscus tiliaceus L. leaves

The present study is aimed to evaluate in vitro thrombolytic activity and antioxidant activity of Hibiscus tiliaceus L. Chemical characterization of bioactive extract was determined by using GC-MS analysis. The thrombolytic potential was performed by the clot lysis method. Antioxidant activity was evaluated by an in vitro assay involving DPPH radical scavenging and hydrogen peroxide scavenging assay. Blood Clots when treated with sterile distilled water, petroleum ether extract, methanol extract and streptokinase showed 7.983 ± 2.197%, 34.10 ± 8.436%, 14.41 ± 5.276 and 62.21 ± 5.519 clot lysis, respectively. Petroleum ether extract shows significant activity when compared with control (P ≤ 0.05). All extracts showed concentration dependent free radical scavenging activity. Bioactive extract of H. tiliaceus contained 23 phytoconstituents as revealed by GC-MS study. The significant thrombolytic activity is exhibited due to presence of sterols as reveled by GC-MS analysis. Further study required to confirm in vivo clot lysis properties.