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CircRNA_0078767 promotes osteosarcoma progression by increasing CDK14 expression through sponging microRNA-330-3p.

Guofeng Zhang1, Yonglin Zhu1, Chengzhen Jin1

  • 1Department of Orthopedics, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, 264100, China.

Chemico-Biological Interactions
|March 21, 2022
PubMed
Summary
This summary is machine-generated.

Circular RNA circ_0078767 promotes osteosarcoma by upregulating CDK14 via the miR-330-3p pathway. This mechanism enhances tumor growth and metastasis, offering potential therapeutic targets for osteosarcoma.

Keywords:
CircRNA_0078767Cyclin-dependent kinase 14InvasionMigrationOsteosarcomaProliferationSpongemicroRNA-330-3p

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNA (circRNA)-associated competing endogenous RNA (ceRNA) networks are crucial in cancer.
  • The specific ceRNA network involving circRNAs, microRNAs (miRNAs), and messenger RNAs in osteosarcoma remains incompletely understood.

Purpose of the Study:

  • To investigate the role of the circ_0078767/miR-330-3p/CDK14 ceRNA network in osteosarcoma.
  • To elucidate the underlying molecular mechanisms driving osteosarcoma progression.

Main Methods:

  • Bioinformatic analysis to identify differentially expressed circRNAs and miRNAs.
  • Experimental validation including RNA immunoprecipitation, RNA pull-down, and dual-luciferase reporter assays.
  • In vitro and in vivo experiments to assess functional roles in osteosarcoma cells and tumor growth.

Main Results:

  • Circ_0078767 was aberrantly expressed in osteosarcoma tissues and cells, correlating with poor prognosis.
  • Circ_0078767 promoted osteosarcoma cell proliferation, invasion, and migration, effects reversed by miR-330-3p.
  • MiR-330-3p directly targeted and downregulated CDK14, inhibiting malignant phenotypes.
  • In vivo studies confirmed circ_0078767 upregulates CDK14 by sponging miR-330-3p, enhancing tumorigenesis and metastasis.

Conclusions:

  • Circ_0078767 acts as an oncogenic circRNA in osteosarcoma by regulating the miR-330-3p/CDK14 axis.
  • This circRNA-mediated ceRNA network plays a significant role in osteosarcoma development and progression.
  • Targeting the circ_0078767/miR-330-3p/CDK14 pathway may offer a novel therapeutic strategy for osteosarcoma.