LINC01140 inhibits nonsmall cell lung cancer progression and cisplatin resistance through the miR-4742-5p/TACC1 axis

Area of Science:

• Molecular Biology
• Oncology
• Gene Regulation

Background:

• Long noncoding RNAs (lncRNAs) are implicated in nonsmall cell lung cancer (NSCLC) progression and drug resistance.
• The specific roles of long intergenic nonprotein coding RNA 01140 (LINC01140) in NSCLC remain to be fully elucidated.

Purpose of the Study:

• To investigate the regulatory functions and underlying mechanisms of LINC01140 in NSCLC progression and chemoresistance.
• To explore the interaction between LINC01140, miR-4742-5p, and transforming acidic coiled-coil 1 (TACC1) in NSCLC.

Main Methods:

• Quantitative real-time PCR and western blot assays were employed to measure gene and protein expression.
• Luciferase reporter and RNA immunoprecipitation assays were utilized to confirm molecular interactions.
• Cellular assays including transwell invasion, flow cytometry, and CCK-8 were performed to assess invasion, apoptosis, and drug sensitivity.

Main Results:

• LINC01140 expression was found to be downregulated in NSCLC, while miR-4742-5p was upregulated.
• LINC01140 acts as a competing endogenous RNA (ceRNA) for miR-4742-5p, thereby positively regulating TACC1 expression.
• Overexpression of LINC01140 suppressed NSCLC cell invasion and cisplatin resistance while promoting apoptosis, effects that were reversed by miR-4742-5p.
• Inhibition of miR-4742-5p mimicked the effects of LINC01140, and TACC1 silencing abrogated these changes.

Conclusions:

• LINC01140 plays an inhibitory role in NSCLC progression and cisplatin resistance.
• The LINC01140/miR-4742-5p/TACC1 axis represents a potential therapeutic target for NSCLC treatment.