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Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

Published on: February 16, 2022

SARS-CoV-2 proteome microarray for COVID-19 patient sera profiling.

Dan-Yun Lai¹, He-Wei Jiang¹, Yang Li²

¹Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai, China.

|March 21, 2022

Summary

This summary is machine-generated.

This study presents a protocol for analyzing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteome immunogenicity using microarrays. This method helps map antibody responses (IgG and IgM) to understand SARS-CoV-2 immunity.

Keywords:

Biotechnology and bioengineering Clinical Protocol Health Sciences Immunology Protein Biochemistry Protein expression and purification Proteomics

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Published on: April 6, 2021

Area of Science:

Immunology
Virology
Biotechnology

Background:

The immunogenic potential of the SARS-CoV-2 proteome remains largely uncharacterized.
Understanding antibody responses is crucial for developing effective vaccines and therapeutics.

Purpose of the Study:

To describe a detailed protocol for analyzing SARS-CoV-2 proteome immunogenicity.
To establish a method for profiling antibody responses (IgG and IgM) against SARS-CoV-2 proteins.

Main Methods:

Development and fabrication of a SARS-CoV-2 proteome microarray.
Expression and affinity purification of viral proteins using E. coli and eukaryotic systems.
Sera profiling to detect specific IgG and IgM antibody responses.

Main Results:

The protocol enables the creation of an antibody response landscape against the SARS-CoV-2 proteome.
Demonstrates a method for comprehensive immunogenicity assessment.

Conclusions:

This protocol provides a valuable tool for deeper understanding of SARS-CoV-2 immunity.
Facilitates research into host-pathogen interactions and immune evasion strategies.