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Updated: Sep 29, 2025

Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane
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Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane

Published on: February 8, 2020

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miR-1251-5p Overexpression Inhibits Proliferation, Migration, and Immune Escape in Clear Cell Renal Cell Carcinoma by

Lin Yue1, Hualong Lin2, Shaofei Yuan2

  • 1School of Nursing, Hunan University of Medicine, Huaihua 418000, China.

Journal of Oncology
|March 21, 2022
PubMed
Abstract

Insights

MicroRNA-1251-5p suppresses clear cell renal cell carcinoma (ccRCC) progression by targeting NPTX2. This finding offers new therapeutic strategies for ccRCC patients, highlighting miR-1251-5p as a potential biomarker.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNA-1251-5p is a known tumor suppressor in various cancers.
  • Its specific role in clear cell renal cell carcinoma (ccRCC) remains unelucidated.

Purpose of the Study:

  • To investigate the function and mechanism of miR-1251-5p in ccRCC.
  • To explore miR-1251-5p as a potential therapeutic target and biomarker for ccRCC.

Main Methods:

  • Utilized TCGA and GEO databases for expression analysis of miR-1251-5p and target genes in ccRCC tissues.
  • Employed cell-based assays (MTT, colony formation, Transwell) and molecular techniques (dual-luciferase reporter, Western blot) to determine functional roles and mechanisms.
  • Bioinformatic analysis identified potential target mRNAs.

Main Results:

  • miR-1251-5p directly targets the 3'-UTR of NPTX2.
  • Lower miR-1251-5p and higher NPTX2 expression were observed in ccRCC tissues compared to normal tissues.
  • Both miR-1251-5p and NPTX2 serve as prognostic biomarkers for ccRCC patients.
  • miR-1251-5p significantly inhibited ccRCC cell proliferation, migration, and immune escape by targeting NPTX2.

Conclusions:

  • miR-1251-5p acts as a tumor suppressor in ccRCC by inhibiting cell proliferation, migration, and immune evasion through NPTX2.
  • These findings provide novel insights into targeted therapy for ccRCC.

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