Transition of Dephospho-DctD to the Transcriptionally Active State via Interaction with Dephospho-IIA^Glc

Exopolysaccharides (EPSs), biofilm-maturing components of Vibrio vulnificus, are abundantly produced when the expression of two major EPS gene clusters is activated by an enhancer-binding transcription factor, DctD₂, whose expression and phosphorylation are induced by dicarboxylic acids. Surprisingly, when glucose was supplied to V. vulnificus, similar levels of expression of these clusters occurred, even in the absence of dicarboxylic acids. This glucose-dependent activation was also mediated by DctD₂, whose expression was sequentially activated by the transcription regulator NtrC. Most DctD₂ in cells grown without dicarboxylic acids was present in a dephosphorylated state, known as the transcriptionally inactive form. However, in the presence of glucose, a dephosphorylated component of the glucose-specific phosphotransferase system, d-IIA^Glc, interacted with dephosphorylated DctD₂ (d-DctD₂). While d-DctD₂ did not show any affinity to a DNA fragment containing the DctD-binding sequences, the complex of d-DctD₂ and d-IIA^Glc exhibited specific and efficient DNA binding, similar to the phosphorylated DctD₂. The d-DctD₂-mediated activation of the EPS gene clusters' expression was not fully achieved in cells grown with mannose. Furthermore, the degrees of expression of the clusters under glycerol were less than those under mannose. This was caused by an antagonistic and competitive effect of GlpK, whose expression was increased by glycerol, in forming a complex with d-DctD₂ by d-IIA^Glc. The data demonstrate a novel regulatory pathway for V. vulnificus EPS biosynthesis and biofilm maturation in the presence of glucose, which is mediated by d-DctD₂ through its transition to the transcriptionally active state by interacting with available d-IIA^Glc. IMPORTANCE Transcription regulation by bacterial two-component systems is achieved by a response regulator upon its transition to the transcriptionally active form via kinase activity of its cognate sensor under specific conditions. A well-known response regulator, DctD, is converted to its phosphorylated form when DctB senses ambient dicarboxylic acids. Phospho-DctD induces expression of its regulon, including the gene clusters for biosynthesis of exopolysaccharides (EPSs), the essential constituents of biofilm matrix. In the absence of dicarboxylic acids, however, DctD-mediated induction of these EPS gene clusters and biofilm maturation was observed if glucose was supplied. This suggests that dephospho-DctD could play a role in activating the transcription of target genes. A component of glucose-phosphotransferase system, IIA^Glc, was present in a dephosphorylated state in the presence of glucose. Dephospho-DctD formed a complex with dephospho-IIA^Glc and was converted to a transcriptionally active state. These findings suggest the other response regulators could also have alternative pathways of activation independent of phosphorylation.