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Related Experiment Video

Updated: Sep 29, 2025

Data Acquisition and Analysis In Brainstem Evoked Response Audiometry In Mice
08:51

Data Acquisition and Analysis In Brainstem Evoked Response Audiometry In Mice

Published on: May 10, 2019

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Protocol for assessing auditory brainstem response in mice using a four-channel recording system.

Lingling Zeng1, Wen Zhou2, Dan Bing1

  • 1Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.

STAR Protocols
|March 22, 2022
PubMed
Summary

This study details a four-channel Auditory Brainstem Response (ABR) recording protocol for mice. This method offers a superior approach for evaluating auditory sensitivity in hearing research.

Keywords:
BehaviorHealth SciencesModel OrganismsNeuroscience

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Area of Science:

  • Neuroscience
  • Auditory research
  • Animal models

Background:

  • Auditory sensitivity evaluation is crucial for hearing impairment research.
  • Auditory Brainstem Response (ABR) recording in mice is a key method for assessing hearing.
  • Traditional techniques for auditory assessment have limitations.

Purpose of the Study:

  • To describe a detailed protocol for recording ABR in mice.
  • To utilize a four-channel recording system for enhanced ABR measurement.
  • To provide a standardized method for auditory sensitivity assessment in rodent models.

Main Methods:

  • Detailed animal preparation procedures.
  • Setup and configuration of a four-channel ABR recording system.
  • Recording of click- and tone burst-evoked ABRs.
  • Data analysis techniques for ABR waveforms.

Main Results:

  • A comprehensive protocol for ABR recording in mice was established.
  • The four-channel system allows for efficient and detailed auditory response capture.
  • The described method facilitates accurate assessment of auditory sensitivity.

Conclusions:

  • The presented protocol provides a robust method for ABR recording in mice.
  • This technique is valuable for researchers studying hearing function and impairment.
  • Standardized ABR recording enhances the reliability of auditory sensitivity data in mice.