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Updated: Sep 29, 2025
Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis
Published on: March 20, 2021
Optimization of Peripheral Blood Mononuclear Cell Extraction from Small Volume of Blood Samples: Potential
Deanira Patrone1, Nicola Alessio1, Nicola Antonucci2
1Department of Experimental Medicine, Division of Molecular Biology, Biotechnology and Histology, University of Campania "Luigi Vanvitelli", via S. Maria di Costantinopoli 16, 80138 Naples, Italy.
Insights
This study presents a simple method for collecting peripheral blood mononuclear cells (PBMC) from children with autism spectrum disorder. This technique aids in studying immune responses and drug toxicity in these children.
Area of Science:
- Pediatric Medicine
- Immunology
- Cell Biology
Background:
- Collecting blood samples from children with autism spectrum disorder (ASD) for medical research presents unique challenges.
- Peripheral blood mononuclear cells (PBMC) are crucial for studying immune responses and drug toxicity.
- Monocytic cells within PBMC play a significant role in inflammatory and immune system alterations.
Purpose of the Study:
- To develop a simple and reliable protocol for isolating peripheral blood mononuclear cells (PBMC) from small blood volumes.
- To facilitate research on immune responses and drug toxicity in children with autism spectrum disorder.
Main Methods:
- Collection of small peripheral blood volumes from pediatric subjects.
- Isolation of peripheral blood mononuclear cells (PBMC) using a straightforward protocol.
Main Results:
- Successful isolation of PBMC from limited blood samples.
- The protocol is demonstrated to be simple and reliable for pediatric applications.
Conclusions:
- The developed protocol offers a viable method for obtaining PBMC from children with autism spectrum disorder.
- This advancement supports further research into the immunological aspects of ASD and potential therapeutic interventions.
Abstract:
Managing medical procedures for children with problematic disorders is a challenging approach, especially in the case of blood withdrawal for autism spectrum disorder-affected children. Peripheral blood mononuclear cells (PBMC) represent an important cellular model to study immune responses and drug toxicity. The monocytic cells, a fraction of PBMC, are strongly involved in some pathophysiological processes, such as inflammation and immune system changes. Here, we propose a simple, reliable protocol for obtaining peripheral blood-derived mononuclear cells from small volumes of blood samples.
