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Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
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DaXi-high-resolution, large imaging volume and multi-view single-objective light-sheet microscopy.

Bin Yang1, Merlin Lange2, Alfred Millett-Sikking3

  • 1Chan Zuckerberg Biohub, San Francisco, CA, USA. bin.yang@czbiohub.org.

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|March 22, 2022
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Summary
This summary is machine-generated.

We developed DaXi, a novel single-objective light-sheet microscope, for fast, high-resolution imaging of large biological samples. This advanced microscopy technique enhances throughput for various research applications.

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Area of Science:

  • Biomedical Engineering
  • Microscopy
  • Optical Imaging

Background:

  • Standard single-objective microscopes offer convenience but lack speed and coverage.
  • Light-sheet microscopy provides speed, resolution, and gentleness but typically requires complex setups.
  • There is a need for microscopy techniques that combine the ease of use of single-objective systems with the advanced capabilities of light-sheet microscopy.

Purpose of the Study:

  • To present DaXi, a novel single-objective light-sheet microscope design.
  • To achieve wider field of view, high-resolution imaging, and fast volumetric acquisition.
  • To enable fuller image coverage for large samples and higher throughput multi-well imaging.

Main Methods:

  • Designed a single-objective light-sheet microscope utilizing oblique plane illumination.
  • Incorporated a custom remote focusing objective for enhanced field of view and resolution.
  • Implemented multi-view imaging and remote coverslip placement for improved coverage and throughput.

Main Results:

  • Achieved lateral resolution of 450 nm and axial resolution of 2 μm.
  • Enabled imaging of large volumes (3,000 × 800 × 300 μm) without compromising image quality.
  • Demonstrated versatility by imaging Drosophila egg chamber development, zebrafish whole-brain activity, and up to nine zebrafish embryos simultaneously.

Conclusions:

  • DaXi successfully combines the advantages of single-objective and light-sheet microscopy.
  • The developed microscope offers significant improvements in speed, resolution, field of view, and throughput.
  • DaXi is a versatile tool for imaging diverse biological systems, facilitating higher throughput research.