Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gas-phase unfolding assay rapidly predicts structure-function relationships in engineered antibodies with tuned flexibilities.

Nature communications·2026
Same author

High-Throughput Screening of Isomeric Reaction Products by Droplet Microfluidics Coupled to Cyclic Ion Mobility-Mass Spectrometry.

Analytical chemistry·2026
Same author

Efficient rhizobium strains enhance nitrogen fixation and growth in alfalfa by improving photosynthetic carbon metabolism and respiratory nitrogen assimilation.

BMC plant biology·2026
Same author

Overcoming Shortcut Learning in RNA-Small Molecule Modeling via Bias-Matched Decoys and Structure-Aware Network Design.

Journal of chemical information and modeling·2026
Same author

Native Charge Detection Mass Spectrometry of Kilobase-Scale Messenger RNAs.

Analytical chemistry·2026
Same author

In vivo evidence for the role of CRFB5 in zebrafish resistance to grass carp reovirus (GCRV) infection.

Developmental and comparative immunology·2026
Same journal

Synthetic Porous Carbons for High-Energy, High-Power Supercapacitors.

Chemical reviews·2026
Same journal

Navigating Misfolded Terrain: ER-Associated Degradation of Membrane Proteins.

Chemical reviews·2026
Same journal

Ink Design for Printing Perovskite Solar Cells and Modules.

Chemical reviews·2026
Same journal

Advanced Single-Atom Catalysts for Thermal-Catalytic C1 Chemistry.

Chemical reviews·2026
Same journal

Copper-Dependent Polysaccharide Monooxygenases: Mechanism and Function.

Chemical reviews·2026
Same journal

To Biotic or Abiotic: Biohybrid Systems for Artificial Photosynthesis.

Chemical reviews·2026
See all related articles

Related Experiment Video

Updated: Sep 29, 2025

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry
08:52

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

Published on: April 6, 2022

3.7K

Mass Spectrometry Methods for Measuring Protein Stability.

Daniel D Vallejo1, Carolina Rojas Ramírez1, Kristine F Parson1

  • 1Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, United States.

Chemical Reviews
|March 22, 2022
PubMed
Summary
This summary is machine-generated.

Mass spectrometry now offers powerful tools to measure protein stability, aiding life science research. These advancements impact drug development and understanding diseases like amyloidosis.

More Related Videos

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders
11:14

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders

Published on: April 14, 2015

16.2K
Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

17.2K

Related Experiment Videos

Last Updated: Sep 29, 2025

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry
08:52

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

Published on: April 6, 2022

3.7K
Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders
11:14

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders

Published on: April 14, 2015

16.2K
Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

17.2K

Area of Science:

  • Biochemistry and Molecular Biology
  • Analytical Chemistry
  • Proteomics

Background:

  • Mass spectrometry is crucial for analyzing cellular molecular composition.
  • It has evolved into a key technology for assessing biomolecular stability, particularly for proteins.
  • Proteins are vital macromolecules involved in cellular functions and serve as drug targets.

Purpose of the Study:

  • To review recent advancements in mass spectrometry techniques for protein stability assessment.
  • To highlight the applications of these techniques in various biological contexts.
  • To discuss the future directions in proteomic-scale stability measurements.

Main Methods:

  • Hydrogen-deuterium exchange (HDX)
  • Chemical cross-linking
  • Ion mobility spectrometry
  • Collision-induced unfolding (CIU)
  • Other proteomic-scale stability assessment techniques

Main Results:

  • Mass spectrometry-based methods provide detailed insights into protein stability.
  • These techniques enable stability assessments on a proteomic scale.
  • Significant impacts observed in studies of membrane proteins, heat shock proteins, amyloidogenic proteins, and biotherapeutics.

Conclusions:

  • Mass spectrometry is a versatile tool for probing protein stability.
  • Recent advancements have expanded its utility in life sciences research.
  • This field is rapidly evolving with significant future potential.