Comamonas fluminis sp. nov., isolated from the Han River, Republic of Korea

A Gram-stain-negative, aerobic and motile bacterial strain, designated CJ34^T, was isolated from Han River water in the Republic of Korea. Strain CJ34^T grew optimally on tryptic soy agar at 30 °C and pH 7.0 in the absence of NaCl. Results of phylogenetic analysis based on 16S rRNA gene sequence showed that strain CJ34^T belonged to the genus Comamonas within the family Comamonadaceae and was most closely related to Comamonas testosteroni ATCC 11996^T and Comamonas thiooxydans DSM 17888^T (both 98.63 % similarity). The average nucleotide identity values between strain CJ34^T and two closely related type strains C. testosteroni ATCC 11996^T and C. thiooxydans DSM 17888^T were 82.77 and 82.73 %, respectively. The major isoprenoid quinone of strain CJ34^T was ubiquinone Q-8. The major cellular fatty acids of strain CJ34^T were C_16 : 0, C_16 : 1  ω6c and/or C_16 : 1  ω7c and C_18 : 1  ω6c and/or C_18 : 1  ω7c. The predominant polar lipids of strain CJ34^T were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified aminophospholipid. Whole genome sequencing revealed that strain CJ34^T had a genome of 4.9 Mbp and the G+C content of the genomic DNA was 59.73 mol%. On the basis of the results of this polyphasic taxonomy study, strain CJ34^T represents a novel species in the genus Comamonas, for which the name Comamonas fluminis sp. nov. is proposed. The type strain is CJ34^T (=KACC 22237^T=JCM 34454^T).