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Isolation and Characterization of Microvesicles from Peripheral Blood
Published on: January 6, 2017
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Microvesicles released from activated CD4+ T cells alter microvascular endothelial cell function
Daria Vdovenko1, Carolina Balbi1,2,3, Dario Di Silvestre4
1Center for Molecular Cardiology, University of Zurich, Schlieren, Switzerland.
European Journal of Clinical Investigation
|March 22, 2022
Summary
Activated T cells release microvesicles that impair endothelial function. These cell-derived microvesicles, enriched in immune proteins, disrupt endothelial barriers and promote inflammation.
Area of Science:
- Immunology
- Cell Biology
- Proteomics
Background:
- Microvesicles are released from cell membranes during activation and apoptosis.
- The function of lymphocyte-derived microvesicles in endothelial health is not well understood.
Purpose of the Study:
- To investigate the proteomic content of microvesicles from activated CD4+ T cells.
- To determine the functional impact of these microvesicles on human microvascular endothelial cells.
Main Methods:
- CD4+ T cells were isolated and stimulated.
- Proteomic profiling of microvesicles (MV.Act and MV.NAct) was performed using LDA and MaxQUANT.
- Functional assays assessed the effects of MV.Act on endothelial cells.
Main Results:
- Stimulated T cells released abundant microvesicles (MV.Act) enriched in immune response and apoptosis-related proteins, including IFN-γ.
- MV.Act induced endothelial superoxide generation, apoptosis, impaired wound healing, and disrupted barrier function.
Conclusions:
- T cell activation releases microvesicles that alter endothelial function and permeability.
- These microvesicles may contribute to tissue inflammation through their effects on endothelial cells.
