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Related Experiment Video

Updated: Sep 29, 2025

Isolation and Characterization of Microvesicles from Peripheral Blood
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Microvesicles released from activated CD4+ T cells alter microvascular endothelial cell function.

Daria Vdovenko1, Carolina Balbi1,2,3, Dario Di Silvestre4

  • 1Center for Molecular Cardiology, University of Zurich, Schlieren, Switzerland.

European Journal of Clinical Investigation
|March 22, 2022
PubMed
Summary
This summary is machine-generated.

Activated T cells release microvesicles that impair endothelial function. These cell-derived microvesicles, enriched in immune proteins, disrupt endothelial barriers and promote inflammation.

Keywords:
T cellsendothelial cellextracellular vesiclemicrovesicleproteomics

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Area of Science:

  • Immunology
  • Cell Biology
  • Proteomics

Background:

  • Microvesicles are released from cell membranes during activation and apoptosis.
  • The function of lymphocyte-derived microvesicles in endothelial health is not well understood.

Purpose of the Study:

  • To investigate the proteomic content of microvesicles from activated CD4+ T cells.
  • To determine the functional impact of these microvesicles on human microvascular endothelial cells.

Main Methods:

  • CD4+ T cells were isolated and stimulated.
  • Proteomic profiling of microvesicles (MV.Act and MV.NAct) was performed using LDA and MaxQUANT.
  • Functional assays assessed the effects of MV.Act on endothelial cells.

Main Results:

  • Stimulated T cells released abundant microvesicles (MV.Act) enriched in immune response and apoptosis-related proteins, including IFN-γ.
  • MV.Act induced endothelial superoxide generation, apoptosis, impaired wound healing, and disrupted barrier function.

Conclusions:

  • T cell activation releases microvesicles that alter endothelial function and permeability.
  • These microvesicles may contribute to tissue inflammation through their effects on endothelial cells.