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SET8 Inhibition Potentiates Radiotherapy by Suppressing DNA Damage Repair in Carcinomas.

Dong Pan1, Ya Rong Du2, Rong Li3

  • 1Department of Radiological Health and Radiation Medicine, School of Public Health and Management, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China;Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences & Key laboratory of Space Radiobiology of Gansu province, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, Gansu, China;Department of Dermatology, Duke University Medical Center, Durham, NC, 27710, USA.

Biomedical and Environmental Sciences : BES
|March 23, 2022
PubMed
Summary

SET8 inhibition enhances cancer radiotherapy by suppressing DNA damage repair. This suggests combining SET8 inhibitors with radiation could improve cancer treatment efficacy and patient outcomes.

Keywords:
CarcinomaDNA repairHistone methylationRadiotherapySET8

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Area of Science:

  • Epigenetics and Cancer Biology
  • Molecular Oncology
  • Radiation Oncology

Background:

  • SET8, a lysine methyltransferase, regulates H4K20me1 and is involved in DNA damage response.
  • The role of SET8 in radiotherapy efficacy remains unclear.
  • Understanding SET8's impact on radiosensitivity is crucial for developing novel cancer therapies.

Purpose of the Study:

  • To determine the efficacy of SET8 inhibition on tumor radiotherapy.
  • To elucidate the underlying mechanisms by which SET8 affects radiosensitivity.
  • To explore the potential of SET8 inhibitors as an adjunct therapy in cancer treatment.

Main Methods:

  • Analysis of clinical data to correlate SET8 expression with radiotherapy benefit.
  • In vivo studies using murine tumor models to assess SET8 deletion effects on radiosensitivity.
  • In vitro experiments measuring apoptosis, DNA damage repair markers (53BP1, γ-H2AX), and micronuclei frequency in SET8-modulated cells.
  • RNA sequencing to identify molecular mechanisms.

Main Results:

  • Low SET8 expression correlated with better radiotherapy outcomes in lung and breast cancer patients.
  • SET8 deletion significantly enhanced tumor radiosensitivity, while overexpression decreased it.
  • SET8 inhibition increased apoptosis and DNA damage, impairing DNA repair kinetics.
  • RNF8 was identified as a factor correlated with SET8's role in DNA damage repair.

Conclusions:

  • SET8 inhibition potentiates radiotherapy by hindering DNA damage repair.
  • Combining SET8 inhibitors with radiation therapy is a promising strategy for precise cancer treatment.
  • Further investigation into SET8 inhibitors is warranted for clinical application in oncology.