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Updated: Sep 29, 2025

Modeling Spontaneous Metastatic Renal Cell Carcinoma mRCC in Mice Following Nephrectomy
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Single-cell RNA-sequencing analysis reveals MYH9 promotes renal cell carcinoma development and sunitinib resistance

Zhipeng Xu1,2, Min Liu3, Jin Wang1,2

  • 1Department of Urology, Shandong Qianfoshan Hospital, Cheeloo college of Medicine, Shandong University, Jinan, Shandong, China.

Cell Death Discovery
|March 23, 2022
PubMed

Insights

Myosin-9 (MYH9) is upregulated in clear cell renal cell carcinoma (ccRCC), driving tumor growth and metastasis. Targeting MYH9 may improve ccRCC patient prognosis and sunitinib treatment response.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genomics

Background:

  • Clear cell renal cell carcinoma (ccRCC) presents significant global health challenges due to tumor heterogeneity, impacting treatment efficacy and patient survival.
  • Single-cell RNA sequencing (scRNA-seq) offers high-resolution insights into the complex genetic landscape of cancers like ccRCC.

Purpose of the Study:

  • To investigate the role of gene expression in ccRCC using scRNA-seq.
  • To identify potential therapeutic targets and prognostic biomarkers for ccRCC.

Main Methods:

  • Profiling gene expression in single cells from human ccRCC and adjacent normal tissues via scRNA-seq.
  • Analyzing the functional impact of MYH9 modulation on ccRCC cell proliferation and metastasis in vitro and in vivo.
  • Investigating the mechanistic role of the MYH9/AKT signaling pathway.

Main Results:

  • MYH9 was commonly upregulated in ccRCC cells and associated with poor patient prognosis.
  • MYH9 knockdown reduced ccRCC cell proliferation and metastasis, while overexpression enhanced these traits.
  • The MYH9/AKT axis was identified as a key pathway influencing ccRCC progression and sunitinib treatment response.

Conclusions:

  • MYH9 is a potential therapeutic target and prognostic predictor for ccRCC.
  • The MYH9/AKT axis influences ccRCC cell behavior and response to sunitinib therapy.
  • Targeting MYH9 offers a promising strategy for improving ccRCC treatment outcomes.

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