Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Sep 29, 2025

A Customizable Chamber for Measuring Cell Migration

A Customizable Chamber for Measuring Cell Migration

Published on: March 12, 2017

Circular gap forming device and two-dimensional area calculation for in vitro cell migration study.

Nannan Zhang¹, Shuyu Fang¹, Yang Bi²

¹Stem Cell Biology and Therapy Laboratory, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Building 7, Room 905, 136 Zhongshan Er Road, Chongqing, 400014, People's Republic of China.

Cell and Tissue Banking

|March 23, 2022

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Pharmacologic inhibition of CSF-1R suppresses intrinsic tumor cell growth in osteosarcoma with CSF-1R overexpression.

Journal of translational medicine·2025

Same author

NMDA Receptors Coordinate Metabolic Reprogramming and Mitophagy in Schwann Cells to Promote Peripheral Nerve Regeneration.

Research (Washington, D.C.)·2025

Same author

The electricity purchasing and selling strategy of load aggregators participating in China's dual-tier electricity market considering inter-provincial subsidies.

Scientific reports·2025

Same author

PU.1 Facilitates Endothelial-to-Mesenchymal Transition in Cardiac Endothelial Cells.

Biology of the cell·2025

Same author

Synergistic Cr doping engineering modulates interfacial built-in electric field and lattice oxygen activation for enhanced electrocatalytic water splitting.

Journal of colloid and interface science·2025

Same author

Characterizing Soil Dissolved Organic Matter Across a Permafrost Thaw Gradient (Continuous to Isolated Patches) in Northeastern China.

Ecology and evolution·2025

This study optimized the cell wound healing assay for better cell migration detection. A new circular wound method using ATRA-treated hepa1-6 cells demonstrated improved reliability and accuracy in measuring cell migration.

Area of Science:

Cell Biology
Biotechnology
Cancer Research

Background:

Cell migration is crucial for understanding biological processes like wound healing and cancer metastasis.
Current scratch assays for in vitro cell migration have limitations in reproducibility and accuracy.
Optimizing cell migration assays is essential for reliable experimental outcomes.

Purpose of the Study:

To develop and validate an optimized cell wound healing assay for precise in vitro cell migration analysis.
To compare the efficacy of a novel circular wound method against traditional linear scratch assays.
To evaluate the effect of ATRA on hepa1-6 cell migration using the optimized assay.

Main Methods:

Hepa1-6 mouse hepatoma cells were treated with ATRA (all-trans retinoic acid).

Keywords:

Cell migration Circular cell wound Wound healing assay

More Related Videos

Quantifying Three-Dimensional Cell Migration Within and Into Granular Hydrogel Biomaterials

Quantifying Three-Dimensional Cell Migration Within and Into Granular Hydrogel Biomaterials

Published on: March 7, 2025

A Tool to Automatically Create Stable and Reproducible Cell-free Gaps for Improving the Reliability of Cell Wound Healing Assay

A Tool to Automatically Create Stable and Reproducible Cell-free Gaps for Improving the Reliability of Cell Wound Healing Assay

Published on: October 4, 2024

Related Experiment Videos

Last Updated: Sep 29, 2025

A Customizable Chamber for Measuring Cell Migration

A Customizable Chamber for Measuring Cell Migration

Published on: March 12, 2017

Quantifying Three-Dimensional Cell Migration Within and Into Granular Hydrogel Biomaterials

Quantifying Three-Dimensional Cell Migration Within and Into Granular Hydrogel Biomaterials

Published on: March 7, 2025

A Tool to Automatically Create Stable and Reproducible Cell-free Gaps for Improving the Reliability of Cell Wound Healing Assay

A Tool to Automatically Create Stable and Reproducible Cell-free Gaps for Improving the Reliability of Cell Wound Healing Assay

Published on: October 4, 2024

A circular gap forming device created standardized 0.2 cm diameter circular wounds in cell monolayers.

Cell migration was observed and quantified by measuring the healing area under microscopy.

Main Results:

The circular wound method allowed for dynamic observation of the entire wound region.
Hepa1-6 cell migration was significantly inhibited by ATRA treatment.
The circular wound method exhibited a smaller standard deviation in wound healing rate compared to linear scratch assays, indicating higher reproducibility.

Conclusions:

The circular cell wound method offers a reliable, accurate, and easy-to-operate alternative for assessing cell migration in vitro.
This optimized assay provides high repetition rates and minimal error, making it suitable for broad laboratory application.
The method is effective in evaluating the impact of substances like ATRA on cell migration dynamics.