Novel ratiometric fluorescent probe based on internal reference and its detection of hydrazine

In this work, dual-emissive ratiometric fluorescent system was constructed by the introduction of an ideal internal reference. By virtue of its unique alkalinity, N₂H₄ could undergo a hydrazinolysis reaction with the ester group of F1, inducing remarkable fluorescence enhancement while the blue fluorescence of the internal reference DPA remained constant. Consequently, the fluorescence intensity ratios (I₅₄₀/I₄₄₀) were proportional to the concentrations of N₂H₄, which was beneficial for the exactly quantitative detection. The skillful strategy granted the sensing system advantages such as relative good solubility in aqueous media, easy-to-design, simple synthesis, large emission shift, good ratiometric response, as well as the successful application in real water samples and cell imaging.