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Isoliquiritigenin Decreases Bone Resorption and Osteoclast Differentiation
Kaitlyn M Joyce1, Carmen P Wong2, Ian A Scriven2
1Botanical Research Center, Department of Food Science and, Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
Molecular Nutrition & Food Research
|March 23, 2022
Summary
Dietary isoliquiritigenin (ILQ) reduces bone resorption in rats and osteoclast differentiation. These effects on bone metabolism appear independent of estrogen receptor signaling.
Area of Science:
- Bone Biology
- Endocrinology
- Nutraceuticals
Background:
- Estrogen depletion significantly impacts bone metabolism.
- Dietary compounds are being investigated for their effects on bone health.
Purpose of the Study:
- To determine if dietary isoliquiritigenin (ILQ) affects bone metabolism in estrogen-depleted rats.
- To investigate if ILQ's effects are mediated through estrogen receptor signaling.
Main Methods:
- A dose-ranging study using ovariectomized rats fed diets with varying ILQ concentrations (0-750 ppm) for 7 days.
- Analysis of bone turnover markers, gene expression in femur and uterus, body composition, and uterine parameters.
- In vitro assessment of ILQ's effect on osteoclast differentiation.
Main Results:
- ILQ administration dose-dependently increased serum ILQ levels but did not alter serum osteocalcin (bone formation marker).
- ILQ significantly reduced serum CTX-1 (bone resorption marker) and tartrate-resistant acid phosphatase expression in osteoclasts.
- Limited overlap was observed between ILQ and endogenous estrogen effects on gene expression; uterine epithelial cell hyperplasia occurred at the highest ILQ dose.
Conclusions:
- Dietary ILQ effectively reduces bone resorption in vivo and osteoclast differentiation in vitro.
- The mechanisms by which ILQ influences bone metabolism appear distinct from classical ovarian hormone actions.
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