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High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease
Published on: October 31, 2012
Use of ELISA for measuring immunoconglutinins
Journal of Immunological Methods
|January 1, 1978
Abstract:
An enzyme-linked immunosorbent assay (ELISA) for detection of immunoconglutinins (IKs) has been developed. IKs are allowed to bind to solid-phase C3 and are then assayed using alkaline phosphatase coupled to anti-IgG or anti-IgM. IK activity was detected in some patients' sera up to 50,000-fold dilution. Significant correlation (r = 0.65) was obtained between the levels of IgM-IKs measured by ELISA and by conventional hemagglutination assay, IgG-IKs and hemagglutination titers did not correlate (r = -0.15).

