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Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

Published on: October 9, 2020

Small RNAs couple embryonic developmental programs to gut microbes.

Hayao Ohno¹, Zhirong Bao¹

¹Developmental Biology Program, Sloan Kettering Institute, New York, NY 10065, USA.

Science Advances

|March 23, 2022

Summary

This summary is machine-generated.

Parental gut microbes can alter worm embryogenesis, leading to changes in cell division and development. This intergenerational information transfer, mediated by microRNAs, challenges the idea of a fixed cell lineage in C. elegans.

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Area of Science:

Developmental biology
Genetics
Microbiology

Background:

Embryogenesis is typically robust to environmental factors.
Parental environmental exposures can potentially influence offspring development.
Proactive developmental changes in response to parental environment are poorly understood.

Purpose of the Study:

To investigate if and how Caenorhabditis elegans embryogenesis changes in response to maternal gut microbes.
To elucidate the mechanisms of intergenerational information transfer during development.

Main Methods:

Studied Caenorhabditis elegans embryogenesis.
Analyzed effects of maternal gut microbes on endodermal cell divisions and morphology.
Investigated the role of the miR-35 microRNA family and the CDC25 pathway.
Examined intergenerational information transmission via endogenous RNA interference.

Main Results:

Harmful maternal gut microbes induce altered endodermal cell divisions and left-right asymmetric development.
Observed double association between intestinal and primordial germ cells.
Demonstrated partial rescue of fecundity.
Identified the miR-35 microRNA family as a key regulator, targeting the CDC25 pathway.

Conclusions:

C. elegans embryogenesis is adaptable to maternal microbial environments.
Challenges the assumption of an invariant cell lineage with a fixed cell number.
Highlights epigenetic control and microRNA-mediated pathways for intergenerational adaptation.