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Updated: Aug 4, 2026
08:48
Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast
Published on: January 26, 2017
Functional complementation between mutations in a yeast suppressor tRNA gene reveals potential for evolution of tRNA
Summary
Mutagenesis of Schizosaccharomyces pombe tRNA revealed that specific mutations can inactivate or restore suppressor function. This highlights tRNA
Area of Science:
- Molecular Biology
- Genetics
- Yeast Biology
Background:
- The sup3-UGA gene in Schizosaccharomyces pombe encodes a tRNA(Ser) suppressor.
- Understanding tRNA gene evolution and function is crucial in molecular biology.
Purpose of the Study:
- To investigate the effects of specific mutations on tRNA suppressor function in Schizosaccharomyces pombe.
- To explore the potential for nucleotide sequence evolution in tRNA genes.
Main Methods:
- Successive rounds of mutagenesis were performed on the sup3-UGA gene.
- Complementation analysis was used to assess suppressor phenotypes.
- Differential gene expression analysis was conducted in Saccharomyces cerevisiae.
Main Results:
- Three mutations (A30, A53, A67) were identified, with varying effects on suppressor activity.
- Mutations A30 and A53 inactivated the suppressor, while A67 was neutral but restored function with A30 or A53.
- Differential expression and RNA processing differences were observed between S. pombe and S. cerevisiae.
Conclusions:
- The study demonstrates significant potential for nucleotide sequence evolution in tRNA genes.
- Divergence in transcriptional and RNA processing levels exists between S. pombe and S. cerevisiae.
- A hierarchy of structural domains in tRNA influences RNase P cleavage.
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07:55A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
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