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Expression, Solubilization, and Purification of Eukaryotic Borate Transporters
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Boron clusters as broadband membrane carriers.

Andrea Barba-Bon1, Giulia Salluce2, Irene Lostalé-Seijo2

  • 1Department of Life Sciences and Chemistry, Jacobs University Bremen, Bremen, Germany.

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Globular dodecaborate clusters, like B12Br12(2-), act as novel inorganic carriers for hydrophilic molecules. These superchaotropic anions facilitate transport across membranes, enabling drug delivery and cellular labeling.

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Area of Science:

  • Biochemistry
  • Materials Science
  • Nanotechnology

Background:

  • Hydrophilic substance membrane translocation is difficult for therapeutics and probes.
  • Conventional amphiphilic carriers can cause aggregation and membrane lysis.
  • Novel carriers are needed to overcome these limitations.

Purpose of the Study:

  • To investigate globular dodecaborate clusters as inorganic membrane carriers.
  • To evaluate the transport capabilities of B12Br12(2-) for hydrophilic cargo.
  • To explore the potential applications of these carriers in cellular delivery.

Main Methods:

  • Utilized liposomal models to assess membrane transport.
  • Characterized cargo types including peptides, amino acids, neurotransmitters, vitamins, antibiotics, and drugs.
  • Conducted mechanistic transport studies to understand carrier activity.
  • Demonstrated cytosolic uptake of bioactive molecules in living cells.

Main Results:

  • Dodeca borate clusters, particularly B12Br12(2-), function as anionic inorganic membrane carriers.
  • Successfully transported a broad range of hydrophilic molecules (146-4,500 Da) across liposomal membranes.
  • Carrier activity correlated with the superchaotropic nature of the cluster anions.
  • Showcased delivery of antineoplastics, proteolysis targeting chimeras, and phalloidin toxin for cytoskeleton labeling in cells.

Conclusions:

  • Globular dodecaborate clusters offer a new class of inorganic membrane carriers.
  • Their superchaotropic properties enable broad hydrophilic cargo delivery.
  • These carriers have potential applications in cell biology, neurobiology, physiology, and pharmaceuticals.