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Circ_0058063 promotes progression of thyroid cancer by sponging miR-330-3p/SDC4 axis

Mingwei Tang1, Fengbo Wang2, Ke Wang2

  • 1Departments of Endocrinology and Metabolism.

Anti-Cancer Drugs
|March 24, 2022
PubMed

Insights

Circular RNA circ_0058063 promotes thyroid cancer (TC) by targeting miR-330-3p and syndecan 4 (SDC4). Silencing circ_0058063 or boosting miR-330-3p inhibits TC cell growth and migration.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are implicated in cancer development.
  • Circ_0058063 is an oncogene in other cancers, but its role in thyroid cancer (TC) is unknown.
  • Understanding circRNA roles is crucial for cancer research.

Purpose of the Study:

  • To investigate the role of circ_0058063 in thyroid cancer (TC).
  • To explore the association between circ_0058063 and miR-330-3p in TC.
  • To elucidate the underlying molecular mechanisms of circ_0058063 in TC progression.

Main Methods:

  • Quantitative reverse transcription PCR for circ_0058063 and miR-330-3p expression.
  • Cell counting kit-8 and scratch adhesion assays for cell proliferation and migration.
  • Dual luciferase reporter and RNA immunoprecipitation assays for interaction analysis.

Main Results:

  • Circ_0058063 was significantly upregulated in TC tissues and cells.
  • miR-330-3p expression was inversely correlated with circ_0058063 levels.
  • Silencing circ_0058063 or upregulating miR-330-3p suppressed TC cell proliferation and migration, affecting Bax and Bcl-2 expression.
  • Circ_0058063 targets miR-330-3p, which in turn targets syndecan 4 (SDC4).

Conclusions:

  • Circ_0058063 acts as an oncogene in thyroid cancer (TC).
  • The circ_0058063/miR-330-3p/SDC4 axis plays a critical role in TC progression.
  • Circ_0058063 promotes TC via the miR-330-3p/SDC4 pathway, representing a potential therapeutic target.

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