Related Experiment Video
Updated: Sep 29, 2025

Multimodal Optical Microscopy Methods Reveal Polyp Tissue Morphology and Structure in Caribbean Reef Building Corals
Published on: September 5, 2014
Comparison of 2D Optical Imaging and 3D Microtomography Shape Measurements of a Coastal Bioclastic Calcareous Sand
Ryan D Beemer1, Linzhu Li2, Antonio Leonti3
1Department of Civil and Environmental Engineering, University of Massachusetts Dartmouth, Dartmouth, MA 02747, USA.
Abstract:
This article compares measurements of particle shape parameters from three-dimensional (3D) X-ray micro-computed tomography (μCT) and two-dimensional (2D) dynamic image analysis (DIA) from the optical microscopy of a coastal bioclastic calcareous sand from Western Australia. This biogenic sand from a high energy environment consists largely of the shells and tests of marine organisms and their clasts. A significant difference was observed between the two imaging techniques for measurements of aspect ratio, convexity, and sphericity. Measured values of aspect ratio, sphericity, and convexity are larger in 2D than in 3D. Correlation analysis indicates that sphericity is correlated with convexity in both 2D and 3D. These results are attributed to inherent limitations of DIA when applied to platy sand grains and to the shape being, in part, dependent on the biology of the grain rather than a purely random clastic process, like typical siliceous sands. The statistical data has also been fitted to Johnson Bounded Distribution for the ease of future use. Overall, this research demonstrates the need for high-quality 3D microscopy when conducting a micromechanical analysis of biogenic calcareous sands.
More Related Videos
Related Concept Videos
Electron Microscope Tomography and Single-particle Reconstruction
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Three-Dimensional Microscopy in Microbiology

