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Related Concept Videos

Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

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Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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Related Experiment Video

Updated: Sep 29, 2025

Fluorescence Lifetime Macro Imager for Biomedical Applications
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A Rapid Method for Detecting Microplastics Based on Fluorescence Lifetime Imaging Technology (FLIM).

Fang Zhou1, Xin Wang1, Guangxin Wang1

  • 1The Analysis and Testing Center, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.

Toxics
|March 24, 2022
PubMed
Summary

This study introduces a novel method for identifying microplastics using fluorescence lifetime imaging and phasor analysis. This technique offers a rapid and accurate way to detect microplastics in complex environmental samples.

Keywords:
fingerprint libraryfluorescence lifetimemicroplasticsphasor analysis

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Area of Science:

  • Environmental Science
  • Analytical Chemistry
  • Spectroscopy

Background:

  • Microplastic pollution is a growing global concern due to its accumulation in ecosystems and potential harm to organisms and humans.
  • Current methods for microplastic detection lack standardization, hindering rapid and accurate identification.
  • There is a need for advanced techniques to effectively analyze microplastics in complex environmental matrices.

Purpose of the Study:

  • To develop and validate a novel method for rapid and accurate microplastic detection using fluorescence lifetime imaging technology.
  • To establish unique phasor fingerprints for different microplastics for identification and localization.
  • To assess the feasibility of the developed method in complex environmental samples with potential interferents.

Main Methods:

  • Utilized fluorescence lifetime imaging technology combined with phasor analysis to obtain unique phasor fingerprints of microplastics.
  • Investigated both Nile red-stained and unstained microplastics.
  • Tested the method's robustness by analyzing microplastics in the presence of simulated environmental interferents like SiO2, chitin, DBDPE, and surface sediments.

Main Results:

  • Obtained unique phasor fingerprints for four types of microplastics.
  • Demonstrated that using autofluorescence phasor fingerprints provided better distinction of microplastics in mixed samples compared to Nile red staining.
  • Successfully identified and located different microplastics in mixed samples and simulated complex environmental matrices.

Conclusions:

  • Fluorescence lifetime imaging with phasor analysis offers a powerful tool for rapid and intuitive identification and localization of microplastics.
  • Autofluorescence-based phasor fingerprinting is a promising approach for microplastic analysis, outperforming traditional staining methods in complex samples.
  • The developed method shows significant potential for real-world applications in environmental monitoring and microplastic analysis.