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Label-free, High-Resolution 3D Imaging and Machine Learning Analysis of Intestinal Organoids via Low-Coherence Holotomography
Published on: August 12, 2025
531
Present Application and Perspectives of Organoid Imaging Technology
Keyi Fei1, Jinze Zhang1, Jin Yuan1
1State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Sun Yat-Sen University, Guangzhou 510060, China.
Bioengineering (Basel, Switzerland)
|March 24, 2022
Summary
Organoid research relies on imaging technologies for monitoring development and function. This review details current and future imaging methods to aid biologists in selecting optimal techniques for organoid studies.
Area of Science:
- Biomedical Engineering
- Developmental Biology
- Cell Biology
Background:
- Organoids are advanced in vitro models mimicking organ structure and function.
- Their applications span growth, disease modeling, drug screening, and cell therapy.
- Direct monitoring of organoid cultures is crucial for data acquisition.
Purpose of the Study:
- To review imaging technologies applicable to organoid research.
- To discuss the principles, advantages, and limitations of existing methods.
- To provide insights into emerging imaging techniques for organoids.
Main Methods:
- Literature review of imaging technologies used in organoid research.
- Analysis of principles, benefits, and drawbacks of various imaging modalities.
- Exploration of prospective imaging advancements.
Main Results:
- Imaging offers direct observation and quantitative analysis of organoids.
- Established techniques provide essential morphological and functional data.
- Future technologies promise enhanced resolution and dynamic monitoring capabilities.
Conclusions:
- Selecting appropriate imaging techniques is vital for successful organoid research.
- This review guides biologists in choosing suitable methods.
- Advancements in imaging systems will further support organoid development and application.
