[Immunoenzyme analysis of monoclonal antibodies on solid-phase infected cells]

Monoclonal MAK-14-7 antibodies to the surface antigen of Venezuelan equine encephalomyelitis virus and OKA series to vaccinia virus antigens were studied by enzyme-immunoassay (EIA) on the solid phase of the infected Vero, BHK-21, and HeLa cells. The immunoglobulins under study from the culture and ascitic fluids of hybridomas were shown to bind specifically with the appropriate antigens of the infected cells. The activity of monoclonal antibodies to viral antigens in EIA on the solid phase of the infected cells was 10-fold higher than in indirect immunofluorescence test. The method has a number of useful advantages such as the simplicity of preparation of the antigen solid phase, rapidly obtainable results, long-term preservation of ready panels with the specific antigen at room temperature. The high sensitivity of the method makes it effectively useful for screening of hybrid clones producing monoclonal antibodies.