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A hyperbranched polyglycerol-functionalized polymer polar stationary phase.

Huiliang Geng1, Zihan Wang1, Feifang Zhang1

  • 1Engineering Research Center of Pharmaceutical Process Chemistry, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology, 130 Meilong RD, Pharmacy School, East-China Univ. Sci. Tech., Shanghai 200237, China.

Journal of Chromatography. A
|March 24, 2022
PubMed
Summary

A new polymer stationary phase using hyperbranched polyglycerol was developed for hydrophilic interaction chromatography (HILIC). This advanced material offers excellent separation of polar compounds and robust performance across a wide pH range.

Keywords:
Hydrophilic interaction liquid chromatographyHyperbranched polyglycerolPolymerStationary phaseSurface-initiated ring-opening polymerization

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Area of Science:

  • Chromatography
  • Polymer Chemistry
  • Materials Science

Background:

  • Hydrophilic interaction chromatography (HILIC) is crucial for separating polar analytes.
  • Development of robust and versatile HILIC stationary phases remains an active research area.
  • Existing HILIC phases can suffer from limited pH stability or high bleed levels.

Purpose of the Study:

  • To synthesize and characterize a novel polymer polar stationary phase functionalized with hyperbranched polyglycerol for HILIC.
  • To evaluate the separation performance and stability of the new HILIC phase.
  • To demonstrate the tunability of functional group capacity through layer-by-layer polymerization.

Main Methods:

  • Surface-initiated ring-opening polymerization of hyperbranched polyglycerol onto hydrolyzed poly(glycidyl methacrylate-divinylbenzene) microspheres.
  • Characterization of the functionalized stationary phase.
  • Evaluation of chromatographic performance using polar analytes under various pH conditions and gradient elution.

Main Results:

  • Successful synthesis of a HILIC stationary phase with hyperbranched polyglycerol.
  • Demonstrated typical HILIC characteristics and good separation performance for polar analytes.
  • Exhibited a wide pH tolerance range (pH 2-12) and negligible bleed level, even with high water content in the mobile phase.

Conclusions:

  • The developed hyperbranched polyglycerol-functionalized stationary phase is a promising material for HILIC applications.
  • The phase offers enhanced stability and performance compared to conventional HILIC materials.
  • The modular synthesis allows for tuning of the phase's properties for specific chromatographic needs.