Related Experiment Video
Updated: Sep 29, 2025
Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice
Published on: December 4, 2020
Incidence of Vancomycin-Resistant Staphylococcus aureus Strains among Patients with Urinary Tract Infections
Samy Selim1, Osama Ahmed Faried2, Mohammed S Almuhayawi3
1Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka 72341, Saudi Arabia.
Abstract:
There has been a substantial rise in the number of vancomycin-resistant Staphylococcus aureus (VRSA) strains during the last several years. The proportion of vancomycin-resistant strains among isolated S. aureus has risen steadily in recent years, with the first spike occurring in critical care units and thereafter in general hospital wards. S. aureus isolates from urinary tract infection patients were studied for their prevalence and antibiotic resistance. From 292 urine samples, 103 bacterial strains (35.3%) were identified as S. aureus. Various antibiotics were used to test the isolates' antibacterial resistance profiles. Antibiotic resistance to erythromycin was found in most bacterial isolates, whereas tobramycin antibiotic sensitivity was found in most of them. Vancomycin resistance was found in 23 of all S. aureus isolates in this study. Analysis for β-lactamase found that 71% of S. aureus isolates were positive in all isolates. There was a single plasmid with a molecular weight of 39.306 Kbp in five selected VRSA isolates that was subjected to plasmid analysis. There was evidence of vancomycin resistance among the S. aureus isolates collected from UTI patients in this investigation. This vancomycin resistance pretenses a challenge in the treatment of S. aureus infections and the need to precisely recognize persons who require last-resort medication such as tobramycin.
Insights
Vancomycin-resistant Staphylococcus aureus (VRSA) is a growing concern. This study found significant vancomycin resistance in S. aureus from urinary tract infections, highlighting treatment challenges.
Area of Science:
- Microbiology
- Infectious Diseases
- Clinical Science
Background:
- Increasing prevalence of vancomycin-resistant Staphylococcus aureus (VRSA) strains globally.
- VRSA emergence in critical care units and general hospital wards poses a significant public health threat.
- Need for understanding antibiotic resistance patterns in S. aureus, particularly in urinary tract infections (UTIs).
Purpose of the Study:
- To investigate the prevalence and antibiotic resistance profiles of Staphylococcus aureus isolates from UTI patients.
- To identify the occurrence of vancomycin resistance in S. aureus from urine samples.
- To analyze the characteristics of vancomycin-resistant S. aureus (VRSA) isolates.
Main Methods:
- Collection and analysis of 292 urine samples to identify S. aureus isolates.
- Antibiotic susceptibility testing using various antibiotics to determine resistance patterns.
- Plasmid analysis was performed on selected VRSA isolates.
Main Results:
- 103 S. aureus strains (35.3%) were identified from urine samples.
- High resistance to erythromycin and 71% of isolates were positive for beta-lactamase.
- Vancomycin resistance was detected in 23 of the S. aureus isolates (22.3%).
- A single plasmid of 39.306 Kbp was identified in five selected VRSA isolates.
Conclusions:
- Evidence of vancomycin resistance in S. aureus isolates from UTI patients.
- Vancomycin resistance in S. aureus presents a significant challenge for treating infections.
- The need for accurate identification of patients requiring last-resort antibiotics like tobramycin is emphasized.
Related Concept Videos
01:26Urine Studies II: Urine Culture and Sensitivity Test
01:25Urinary Tract Infection II: Pathophysiology
01:30Urinary Tract Infection III: Diagnostic Studies and Interprofessional Care
01:26Urinary Tract Infection I: Introduction
01:17Urinary Tract Infection IV: Nursing Management
01:28Acute Pyelonephritis II: Diagnostic Studies and Management