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Updated: Sep 29, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Multiple Sclerosis and Microbiome.
Jana Lizrova Preiningerova1, Zuzana Jiraskova Zakostelska2, Adhish Srinivasan1
1Department of Neurology and Centre of Clinical Neuroscience, First Medical Faculty, Charles University and General Medical Hospital in Prague, 12800 Prague, Czech Republic.
The gut microbiome influences multiple sclerosis (MS) development and progression. Understanding gut-brain axis interactions is key for novel MS therapeutic strategies targeting the microbiome.
Area of Science:
- Neuroimmunology
- Microbiome Research
- Autoimmune Diseases
Background:
- The gut-brain axis and microbiota composition are implicated in various diseases.
- Multiple sclerosis (MS) etiology is complex, with potential gut-brain axis involvement.
Purpose of the Study:
- To explore the relationship between gut microbiota and MS pathophysiology.
- To review germ-free mouse studies on MS and microbiome modulation.
Main Methods:
- Literature review focusing on gut microbiota and MS.
- Analysis of germ-free mouse models in MS research.
Main Results:
- Gut microbiota composition is linked to MS pathology.
- Germ-free mouse studies provide insights into MS mechanisms.
Conclusions:
- Microbiome modulation presents potential therapeutic avenues for MS.
- Further research is needed on microbiota's role in MS patient response to treatments.
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