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A new liquid medium without blood and serum for culture of hemoflagellates
The American Journal of Tropical Medicine and Hygiene
|September 1, 1986
Abstract:
A liquid medium without blood or serum was developed for cultivation of hemoflagellates. To a basic LIT medium containing liver infusion broth and tryptose, a mixture of RPMI 1640 and Medium 199 was added. This combination permitted high parasite yields useful for biochemical and immunological studies.
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