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Related Concept Videos

FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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In-situ Hybridization02:31

In-situ Hybridization

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In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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Updated: Sep 29, 2025

Visualization of Candida albicans in the Murine Gastrointestinal Tract Using Fluorescent In Situ Hybridization
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Identification of Mucormycosis by Fluorescence In Situ Hybridization Targeting Ribosomal RNA in Tissue Samples.

Jill Jasmine Dalimot1,2, Ilka Mc Cormick Smith1, Jasmin Gerkrath1

  • 1Mycotic and Parasitic Agents and Mycobacteria, Department of Infectious Diseases, Robert Koch Institute (RKI), 13353 Berlin, Germany.

Journal of Fungi (Basel, Switzerland)
|March 25, 2022
PubMed
Summary

Fluorescence in situ hybridization (FISH) targeting Mucorales ribosomal RNA (rRNA) shows potential for diagnosing mucormycosis in tissue. However, autofluorescence and other factors may limit its diagnostic accuracy.

Keywords:
fluorescencehybridizationin situmucormycosispathology

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Area of Science:

  • Mycology
  • Infectious Diseases
  • Molecular Diagnostics

Background:

  • Mucormycosis is a severe fungal infection with high mortality.
  • Delayed diagnosis and challenges in identifying Mucorales in tissue hinder effective treatment.
  • Current diagnostic methods, like fungal cultures, often fail to detect Mucorales.

Purpose of the Study:

  • To evaluate the utility of fluorescence in situ hybridization (FISH) for diagnosing mucormycosis.
  • To develop and validate a specific FISH probe for Mucorales identification in tissue samples.

Main Methods:

  • Generated a specific probe combination targeting Mucorales ribosomal RNA (rRNA).
  • Validated probe specificity in silico and with cultivated fungi.
  • Applied rRNA FISH to tissue samples from a mouse model and mucormycosis patients.

Main Results:

  • Mucorales hyphae showed a distinct cytoplasmic signal in mouse model tissues.
  • A positive FISH signal was detected in 58.3% of patient samples (7/12).
  • Autofluorescence in 42.9% of positive samples (3/7) reduced diagnostic yield; nutrient availability and antifungal therapy may affect signal intensity.

Conclusions:

  • rRNA FISH can aid mucormycosis diagnosis in a subset of cases.
  • FISH signal intensity may correlate with the physiological state of Mucorales.
  • Further research is required to establish the definitive role of FISH in diagnosing mucormycosis from various clinical samples.