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Updated: Sep 29, 2025

Confocal Laser Scanning Microscopy of Calcium Dynamics in Acute Mouse Pancreatic Tissue Slices

Confocal Laser Scanning Microscopy of Calcium Dynamics in Acute Mouse Pancreatic Tissue Slices

Published on: April 13, 2021

Calcium Signaling in Pancreatic Immune Cells In situ.

Oleksiy Gryshchenko^1,2, Julia V Gerasimenko¹, Ole H Petersen¹

¹Cardiff School of Biosciences, Cardiff University, Cardiff CF10 3AX, UK.

Function (Oxford, England)

|March 25, 2022

Summary

Immune cells, likely macrophages, in mouse pancreas show calcium (Ca2+) signals primarily from ATP. Acute pancreatitis increases these cells, suggesting their role in inflammation.

Keywords:

ATP P2Y receptors acute pancreatitis calcium signaling exocrine pancreas pancreatic acinar cells pancreatic immune cells pancreatic lobules pancreatic macrophages pancreatic stellate cells

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Preparation of Pancreatic Acinar Cells for the Purpose of Calcium Imaging, Cell Injury Measurements, and Adenoviral Infection

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Imaging Calcium Dynamics in Subpopulations of Mouse Pancreatic Islet Cells

Imaging Calcium Dynamics in Subpopulations of Mouse Pancreatic Islet Cells

Published on: November 26, 2019

Area of Science:

Immunology
Cellular Physiology
Gastroenterology

Background:

Pancreatic immune cells, primarily macrophages, are sparsely distributed in normal pancreas.
Acute pancreatitis (AP) significantly increases the density of these immune cells.

Purpose of the Study:

To characterize calcium (Ca2+) signaling in pancreatic immune cells.
To investigate the role of these signals in acute pancreatitis.

Main Methods:

Live mouse pancreatic lobules were used to record Ca2+ signals in immune cells, acinar cells, and stellate cells.
Immunochemistry identified immune cells as macrophages.
Pharmacological agents, including purinergic agonists and antagonists, were employed.

Main Results:

ATP was the main stimulus for Ca2+ signals in pancreatic immune cells, also responding to acetylcholine and bradykinin.
ATP-induced signals involved P2Y1 and P2Y13 receptors, primarily releasing Ca2+ from internal stores and subsequent store-operated Ca2+ entry.
These Ca2+ signals were dependent on IP3 generation and IP3 receptors.

Conclusions:

Pancreatic immune cells, identified as macrophages, exhibit significant ATP-evoked Ca2+ signaling.
The increased presence and signaling of these cells during acute pancreatitis suggest a crucial role in the inflammatory response to acinar cell injury.