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Related Concept Videos

Overview of Exosomes01:36

Overview of Exosomes

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Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
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Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles
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Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

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Exosome Processing and Characterization Approaches for Research and Technology Development.

James J Lai1, Zoe L Chau1, Sheng-You Chen2

  • 1Department of Bioengineering, University of Washington, Seattle, WA, 98195, USA.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|March 25, 2022
PubMed
Summary
This summary is machine-generated.

Manufacturing high-quality exosomes is key for their use as biomarkers and therapeutics. This review compares exosome processing and characterization methods to guide research and development for these extracellular vesicles.

Keywords:
Good Manufacturing PracticesMISEV2018 guidelinesanalytical characterizationsexosome clinical trialsexosomesextracellular vesiclesisolation processes

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Area of Science:

  • Biotechnology
  • Biomedical Research
  • Extracellular Vesicles

Background:

  • Exosomes, vesicles from parent cells, show promise as disease biomarkers and therapeutic agents.
  • Standardized Good Manufacturing Practices are lacking for exosome processing and characterization.
  • A need exists for well-characterized reference exosome materials for method selection.

Purpose of the Study:

  • To compare exosome processing and characterization methods.
  • To recommend exosome workflows for research and technology development.
  • To provide an introduction to exosome properties, biological roles, and clinical trials.

Main Methods:

  • Literature review of exosome processing techniques.
  • Comparative analysis of exosome characterization methods.
  • Summary of current clinical trials involving exosomes.

Main Results:

  • Identified variations in exosome isolation, purification, and analysis methods.
  • Highlighted the absence of standardized protocols and reference materials.
  • Detailed physical, chemical, and biological properties of exosomes.

Conclusions:

  • Standardization of exosome manufacturing is crucial for clinical applications.
  • Comparative analysis aids in selecting appropriate methods for exosome research.
  • Exosomes hold significant potential in diagnostics and therapeutics.