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Isolation of Murine Embryonic Hemogenic Endothelial Cells
08:56

Isolation of Murine Embryonic Hemogenic Endothelial Cells

Published on: June 17, 2016

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Hemogenic and aortic endothelium arise from a common hemogenic angioblast precursor and are specified by the Etv2

Shizheng Zhao1, Shachuan Feng1, Ye Tian1

  • 1Division of Life Science, State Key Laboratory of Molecular Neuroscience, Center of Systems Biology and Human Health, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong 999077, China.

Proceedings of the National Academy of Sciences of the United States of America
|March 25, 2022
PubMed

SignificanceHematopoietic stem cells (HSCs) are generated from specialized endothelial cells, called hemogenic endothelial cells (HECs). It has been debated whether HECs and non-HSC-forming conventional endothelial cells (cECs) arise from a common precursor or represent distinct lineages. Moreover, the molecular basis underlying their distinct fate determination is poorly understood. We use photoconvertible labeling, time-lapse imaging, and single-cell RNA-sequencing analysis to trace the lineage of HECs. We discovered that HECs and cECs arise from a common hemogenic angioblast precursor, and their distinct fate is determined by high or low dosage of Etv2, respectively. Our results illuminate the lineage origin and a mechanism on the fate determination of HECs, which may enhance the understanding on the ontogeny of HECs in vertebrates.

Keywords:
endotheliumfate determinationhemogenic endotheliumlineage origin

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